Gibco™ Viral Production Cells 2.0

Catalog No.	Quantity
A51218	6 x 1 mL
A49784	1 mL

Catalog No. A51218 Supplier Gibco™ Supplier No. A51218

Description

Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.

Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.

• Adapted to the chemically defined Viral Production Medium and maintained in suspension culture in that medium
• Highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
• Supplied frozen in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL in Viral Production Medium and 10% DMSO
• Should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance
• Clonal, 293F-derived, high-producer cell line
• Absence of SV40 large T antigen
• Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
• Enable production of high AAV titers, greater than 5 x 10¹⁰ vg/mL (unpurified)
• Robust scalability and passage stability

Specifications

• Cell State: Frozen
• Concentration: 1e7 cells/mL
• Content And Storage: 6 cryovials, store in vapor phase liquid nitrogen
• Cell Line: Viral Production Cells 2.0
• Cell Type: Kidney (Embryonic)
• Culture Type: Suspension Cell Culture
• Form: Frozen
• Species: Human
• Format: 6 Vials
• For Use With (Application): AAV Production
• Media Recommendation: Gibco Viral Production Medium
• No. of Cells: 1 x 10⁷ cells
• Product Line: AAV-MAX
• Product Type: Viral Production Cells
• Quantity: 6 x 1 mL
• Shipping Condition: dry ice for domestic shipment; liquid nitrogen for international shipment
• Source: Human

Frequently Asked Questions (FAQs)

Can Viral Production Cell 2.0 (Cat. No. A49784, A51218) only grow in suspension, or can they be adherent using specific medium conditions?

Viral Production Cells 2.0 were designed specifically for suspension cultures. We did not validate their usage with adherent culture and therefore do not recommend doing so.

Can I use Viral Production Cells (Cat. No. A35347, A35827) with the AAV MAX Helper-Free AAV Production System?

The Viral Production Cells 2.0 are specifically designed to work with the other components of the AAV MAX Helper-Free AAV Production System to provide maximal adeno-associated virus (AAV) titers. Viral Production Cells could be used but we don't recommend doing this as it will reduce system performance.

What is the highest density that Viral Production Cells 2.0 can be cultured at?

The Viral Production Cells 2.0 achieve maximum cell densities of >1.2 x 10E7 cells/mL in shake flask cultures.

What is the passage stability of the Viral Production Cells 2.0?

We recommend using the Viral Production Cells 2.0 between passages 3 to 25 for adeno-associated virus (AAV) production.

How long do the Viral Production Cells 2.0 require to recover post-thaw before transfection?

We recommend allowing freshly thawed Viral Production Cells 2.0 to recover in culture for at least three passages prior to transfection.

Are the Viral Production Cells 2.0 a clonal cell line?

Yes, the Viral Production Cells 2.0 cells are a clonal cell line derived from single cell cloning of 293-F cells.

For Research Use Only. Not for use in diagnostic procedures.