Gibco™ Viral Production Medium, AGT™

Catalog No.	Reconstituted Volume
A5147101	1 L
A5147102	10 L
A5147103	50 L
A5147104	100 L

Catalog No. A5147101 Supplier Gibco™ Supplier No. A5147101

Description

Gibco Viral Production Medium, AGT, is the dry-format version of CTS Viral Production Medium, a chemically defined, serum-free, protein-free, animal origin-free, phenol red-free medium.

• Gibco Viral Production Medium, AGT is the dry-format version of CTS Viral Production Medium, a chemically defined, serum-free, protein-free, animal origin-free, phenol red-free medium
• Medium is part of the CTS AAV-MAX Helper-Free AAV Production System for high-titer adeno-associated virus (AAV) production
• The CTS AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production with research-grade and GMP versions to enable seamless transition from research to commercial manufacturing
• Other components of the CTS AAV-MAX system include CTS Viral Production Cells 2.0, CTS AAV-MAX Transfection Kit, and CTS Viral-Plex Complexation Buffer.

Features of Viral Production Medium, AGT, include:

• Supports growth and transfection of CTS Viral Production Cells 2.0 from shake flask to bioreactor scales
• Helps enable production of high AAV titers, greater than 5 x 10¹⁰ vg/mL (unpurified)
• Simplifies transfer to manufacturing scale due to Advanced Granulation Technology (AGT) enabling auto-pH and simplified dissolution
• Formulated with GlutaMAX Supplement, no further supplementation required
• Manufactured in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to ISO 13485. Drug Master File and Regulatory Support File available upon request.

Advanced Granulation Technology

• Advanced Granulation Technology (AGT) helps simplify medium preparation at large scale with fast rehydration
• A just-add water protocol, and low dust generation, creates less pressure build-up during sterile filtration
• For more information on AGT media preparation at large scale, see Hydration and scale-up of AGT medium in HyPerforma and imPULSE Single-Use Mixers

Specifications

• Cell Line: Viral Production Cells 2.0
• Cell Type: HEK 293 cells
• Concentration: 25.6 g/L
• Form: Powder (AGT)
• Product Type: AAV Production Medium
• Sterility: Non-sterile
• With Additives: Glucose, GlutaMAX
• Without Additives: No Phenol Red, No Antibiotics
• For Use With (Application): AAV Production
• Product Line: AAV-MAX
• Quantity: 1 L
• pH: 6.9 to 7.5
• Reconstituted Volume: 1 L
• Content And Storage: 1 L Securitainer. Store at 2°C to 8°C, protected from light.
• Culture Type: Suspension Cell Culture
• Expression System: Mammalian

Frequently Asked Questions (FAQs)

What settings should I use to count my cells using the Vi-CELL XR Cell Counter?

You can refer to page 9 of the AAV-MAX Helper-Free AAV Production System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019619_AAV-MAX_Helper-Free_AAV_ProductionSystem_UG.pdf) for detailed Vi-CELL XR settings. We recommend diluting cell count samples with PBS to eliminate precipitate artifacts that may be created by interaction with the Vi-CELL reagents.

Does Viral Production Medium, AGT contain a surfactant? Do I need to add extra surfactant for culturing CTS VPC2.0 in bioreactors ?

While the full formulation for Viral Production Medium, AGT is proprietary information that cannot be disclosed, we can confirm that the medium contains a surfactant and that no additional surfactant would need to be added to the medium to support your suspension CTS VPC2.0 in 2 L bench-top bioreactor to 50 L bioreactor.

What is the concentration of glucose in Viral Production Medium, AGT?

Viral Production Medium, AGT is formulated to contain glucose at a concentration of 6 g/L.

What type of filter is recommended to prepare liquid aseptic media from Viral Production Medium, AGT powder?

Viral Production Medium, AGT powder needs to be reconstituted in deionized distilled water or Water For Injection (WFI) for Cell Culture (Cat. No. A12873) and then immediately sterilized by membrane filtration (positive pressure recommended). A low protein binding filter (such as PVDF) with a pore size of 0.2 µM is recommended.

How does Viral Production Medium, AGT powder differ from the research-grade Viral Production Medium (Cat. No. A48179)?

GlutaMAX Supplement (Cat. No. 35050061) is already present in the one-part Viral Production Medium, AGT powder. Once rehydrated, the formulations of the two products are identical provided the research-grade Viral Production Medium (Cat No. A48179) has been supplemented with GlutaMAX Supplement at point of use.

How much Viral Production Medium, AGT powder do I weigh out for one liter of media?

25.6 grams of Viral Production Medium, AGT powder is required for 1 L of media. We do not advise preparing less than 1 L at a time to ensure consistent weighing and rehydration process.