Invitrogen™ ViraPower™ HiPerform™ Lentiviral FastTiter™ Gateway™ Expression Kit

Description

The ViraPower™ HiPerform™ Lentiviral FastTiter™ Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit.

The ViraPower™ HiPerform™ Lentiviral FastTiter™ Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen's ViraPower™ HiPerform™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements. In addition, the vector allows for an accurate determination of titer of functional lentivirus in just two days using Emerald Green Fluorescent Protein (EmGFP).

Advantages

• Transient expression
• Short-term experiments
• High-throughput screening
• 2 day titer of functional virus using EmGFP
• Flexible and versatile Gateway™ recombination cloning technology

Key Features

• WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, allows increased transgene expression and cPPT (Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
• SV40 promoter driving expression of EmGFP
• Emerald Green Fluorescent Protein (EmGFP) allows easy determination of Lentiviral titers by flow cytometry and monitor transduced cells.

Kit includes

• pLenti7.3/V5-DEST™ Gateway™ Vector Kit (Cat. No. V534-06)
• 293FT Cell Line (R700-07)
• ViraPower™ Lentiviral Support Kit (ViraPower™ Lentiviral Packaging Mix and Lipofectamine™ 2000)

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Lentiviral
• Promoter: CMV
• Product Type: Lentiviral Expression Kit
• Reporter Gene: GFP (EmGFP)
• Selection Agent (Eukaryotic): None
• Content And Storage:

  pLenti7.3/V5-DEST™ Gateway™ Vector Kit
  • pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL
  • pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL) , 20 μlL
  Store at -20°C.
  
  ViraPower™ Lentiviral Support Kit
  Contains enough reagents for 20 transfections in 10 cm tissue culture dishes:
  • Lyophilized Packaging Mix, 195 μg, store at -20°C
  • Lipofectamine™ 2000, 0.75 mL, store at 4°C
  
  One Shot™ Stbl3™ chemically competent E. coli box
  • One Shot™ Competent Cells, 20 single-use aliquots of 50 μL
  • S.O.C. medium
  • pUC19 supercoiled plasmid control
  Store at -80°C.
  
  293FT cell line
  • 3 x 10⁶ frozen cells in 1 mL of Freezing Medium, store in liquid nitrogen.

• Special Elements: WPRE, cPPT
• Protein Tag: V5 Epitope Tag
• Cloning Method: Gateway
• Concentration: 6 μg/μL
• Quantity: 1 Kit
• Vector: pLenti
• Product Line: FastTiter™, Gateway™, HiPerform™, ViraPower™
• For Use With (Application): Reporter Assays, Viral Expression

For Research Use Only. Not for use in diagnostic procedures.