Invitrogen™ ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit

Catalog No.	Quantity
K533000	1 kit

Catalog No. K533000 Supplier Invitrogen™ Supplier No. K533000

Description

The ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit.

The ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen's™ ViraPower™ HiPerform™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages

• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway™ recombination cloning technology

Key Features

• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selection

Kit includes

• pLenti6.3/V5-DEST™ Gateway™ Vector Kit (V533-06)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)

Specifications

• Content And Storage:

  Vector kit
  • pLenti6.3/V5-DEST™ (150 ng/μL), 40 μL, store at -20°C
  • pLenti6.3/V5-GW/lacZ Control vector (0.5 μg/μL), 20 μL, store at -20°C
  • One Shot™ Stbl3™ Chemically Competent E. coli, 21 x 50 μL, store at -80°C
  • pUC19 control DNA (10 pg/μL), 50 μL, store at -80°C
  • S.O.C. Medium, 6 mL, store at -80°C
  
  Support kit
  • ViraPower™ Packaging Mix, 195 μg, store at -20°C
  • Lipofectamine™ 2000, 0.75 mL, store at 4°C
  • Blasticidin, 50 mg, store at -20°C
  • 293 FT cells (3 x 10⁶ cells/mL), 1 mL, store in liquid nitrogen

• Cloning Method: Gateway
• Constitutive or Inducible System: Constitutive
• Delivery Type: Lentiviral
• Cell Type: 293-FT
• Format: Kit
• Promoter: CMV
• Vector: pLenti, TOPO-TA Vector
• For Use With (Equipment): HiPerform™, TOPO ™ , ViraPower™
• For Use With (Application): Protein Expression
• Key Functions: Viral Expression
• Product Line: Gateway, HiPerform, ViraPower
• Product Type: Expression Kit
• Protein Tag: V5 Epitope Tag
• Quantity: 1 kit
• Selection Agent (Eukaryotic): Blasticidin
• Special Elements: WPRE, cPPT

For Research Use Only. Not for use in diagnostic procedures.