Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit

Description

The ViraPower HiPerform T-REx Gateway Vector Kit contains the Gateway-adapted ViraPower HiPerform tetracycline-regulated lentiviral expression vector, pLenti6.3/TO/V5-DEST for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells.

The ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ tetracycline-regulated lentiviral expression vector, pLenti6.3/TO/V5-DEST™ for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3/TO/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages

• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable, long-term, tetracycline-regulated expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Key Features

• WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
• Blasticidin selection marker for stable selection under control of SV40 promoter

Kit includes

• pLenti6.3/TO/V5-DEST™ vector
• pLenti3.3/TR vector
• pLenti6.3/TO/V5-GW/lacZ plasmid
• Tetracycline
• One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
• pENTRTM Gus positive control plasmid.

Specifications

• Constitutive or Inducible System: Inducible
• Delivery Type: Lentiviral
• Inducing Agent: Doxycycline, Tetracycline
• Promoter: CMV/TO
• Product Type: Vector Kit
• Selection Agent (Eukaryotic): Blasticidin
• Content And Storage: ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit
  • pLenti6.3/TO/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg
  • pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg
  • pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg
  • Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C and protect from light.
  Store at -20°C unless otherwise noted
  
  One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
  • Stbl3™ Cells in 6 mL of S.O.C. Medium, 21 x 50 μL
  • pUC19 control DNA (10 pg/μL), 50 μL
  Store at -80°C
  
  pENTR™ Gus positive control plasmid, store at -20°C.
• Antibiotic Resistance Bacterial: Ampicillin (AmpR), Blasticidin (BsdR)
• Cleavage: No Cleavage Site
• Gateway Enzyme: LR Clonase™ II Plus, LR Clonase™ II
• PCR Enzyme: Proof-Reading Polymerase
• Protein Tag Position (to your gene): C-terminal
• Replication Origin: pUC Origin
• Selection Marker Promoter: PGK Promoter
• Selection Marker Eukaryotic: BsdR
• Special Elements: WPRE, cPPT
• Protein Tag: V5 Epitope Tag
• Cloning Method: Gateway
• Quantity: 20 Reactions
• Vector: pLenti
• Product Line: Gateway™, HiPerform™, T-REx™, ViraPower™
• For Use With (Application): Viral Expression

For Research Use Only. Not for use in diagnostic procedures.