Expression Systems Baculovirus Titering Service

Supplier:  Expression Systems 94901

Baculovirus titering is routinely set up on Wednesdays with data delivery Thursday afternoon PST. Samples should be received by 11AM Wednesday to be set up that week.

Accurate titering of recombinant baculovirus is an important step in protein production optimization. A high throughput, flow cytometric assay is utilized to quantitate infectious baculovirus particles produced in insect cell culture, allowing for accurate virus titer determination for multiple conditions in less than 24 hours. The assay detects the cell surface expression of gp64 after a short incubation period allowing for a single round of infection. Virus infected cells are then assessed by flow cytometric analysis for gp64 expression.
Shipping Instructions
• Ship 0.5-1 ml of virus stock (as little as 200 l can be titered with advance notice)
• Ship sterile aliquots in 2 ml screw cap tubes or equivalent. Ship overnight on cold packs.

Catalog No. NC9572387