Invitrogen™ Vitamin D2/D3 Competitive ELISA Kit

Catalog No.	Quantity
EEL150	96 Tests

Catalog No. EEL150 Supplier Invitrogen™ Supplier No. EEL150

Description

ELISA

The Vitamin D (VD) ELISA quantitates VD in serum, plasma and other biological fluids. Principle of the method The Competitive mulispecies ELISA research use only kit is designed to quantitatively measure Vitamin D (VD)independent of species . A Vitamin D standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve.Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Vitamin D is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Vitamin D in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Vitamin D2 and vitamin D3 are two forms of vitamin D, a group of fat-soluble vitamins that play crucial roles in maintaining overall health. Vitamin D2, also known as ergocalciferol, is derived from plant sources. It is produced when ultraviolet (UV) light converts ergosterol, a compound found in certain fungi and plants, into vitamin D2. Vitamin D3, also called cholecalciferol, is synthesized in the skin of humans and animals upon exposure to UVB light from the sun. It is the most biologically active form of vitamin D and is also found in certain animal-based food sources. Both vitamin D2 and D3 undergo similar metabolic processes in the liver and kidneys to be converted into their active form, known as calcitriol. Calcitriol acts as a hormone that regulates calcium and phosphorus metabolism in the body.

Specifications

• Assay Range: 6.25-400 ng/mL
• Assay Sensitivity: 3.75 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: calciferol; Cholecalciferol; Ergocalciferol; Vitamin D2; vitamin D3calciferol; Cholecalciferol; Ergocalciferol; Vitamin D2; vitamin D3;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Vitamin D2/D3
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min