Invitrogen™ Vitamin D3 Competitive ELISA Kit

Catalog No.	Quantity
EEL151	96 Tests

Catalog No. EEL151 Supplier Invitrogen™ Supplier No. EEL151

Description

ELISA

The Vitamin D3 (VD3) ELISA quantitates VD3 in serum, plasma and other biological fluids. Principle of the method The competitive multispecies ELISA research kit is designed to quantitatively measure Vitamin D3, independent of species . A Vitamin D3 standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Vitamin D3 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. A TMB substrate solution is then added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Vitamin D3 in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Vitamin D3, also known as cholecalciferol, is a form of vitamin D that is synthesized in the skin upon exposure to ultraviolet B (UVB) light from the sun. It is the most biologically active form of vitamin D and plays a crucial role in maintaining overall health. Structurally, vitamin D3 is a steroid hormone precursor that undergoes metabolic processes in the liver and kidneys to be converted into its active form, known as calcitriol. Calcitriol acts as a hormone that regulates calcium and phosphorus metabolism in the body. The main function of vitamin D3 is to facilitate the absorption of calcium and phosphorus from the intestine, promoting their utilization for bone health and growth. It aids in the regulation of bone mineralization, ensuring the proper formation and maintenance of strong and healthy bones. Vitamin D3 is also involved in various other physiological processes beyond bone health. It plays a vital role in modulating immune function, helping to regulate the immune response and reduce the risk of autoimmune diseases. Additionally, it has been linked to cardiovascular health, muscle function, and mood regulation. In terms of localization, vitamin D receptors are present in various tissues throughout the body, including the intestines, bones, kidneys, immune cells, and brain. This widespread distribution of vitamin D receptors highlights the importance of maintaining adequate vitamin D3 levels for optimal functioning of these tissues and overall health.

Specifications

• Assay Range: 1.56-100 ng/mL
• Assay Sensitivity: 0.94 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CholecalciferolCholecalciferol;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Vitamin D3
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min