Prussian blue reaction involves the treatment of sections with acid solutions of ferrocyanides. Any ferric ion (+3) present in the tissue combines with the ferrocyanide and results in the formation of a bright blue pigment called Prussian blue. This is one of the most sensitive histochemical tests and will demonstrate even single granules of iron in blood cells. Small amounts of iron are found normally in spleen and bone marrow. Excessive amounts are present in hemochromatosis, with deposits found in the liver and pancreas, hemosiderosis, with deposits in the liver, spleen, and lymph nodes. This kit is designed for staining ferric iron in tissue sections.(A printed user manual is included)VB-3009-1 Potassium Ferrocyanide Solution 125 mlVB-3009-2 Hydrochloric Acid Solution 125 mlVB-3009-3 Nuclear Fast Red Solution 250 ml