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New England Biolabs, Inc. WarmStart Nt.BstNBI-1000 u
Supplier: New England Biolabs, Inc. R0725S
WarmStart Nt.BstNBI is a site specific nicking endonuclease cloned fromBacillus Stereothermophilus. Restriction Enzyme Cut Site GAGTC(4/-5). It is formulated with a reversibly-bound aptamer which inhibits its nicking activity at temperatures below 40C. WarmStart Nt.BstNBI cleaves only one strand of DNA on a double-stranded DNA substrate. The nicking endonuclease catalyzes a single strand break 4 bases beyond the 3side of the recognition sequence.
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