Thermo Scientific™ SureSTART™ WebSeal™ 96-Well Plate Sealing Mats, 7 mm Diameter, Round, Level 2 High-Throughput Applications

Catalog No.	Pre-slit	Material	Format	No. per Pack
03-255-001	No	EVA	Domed Base	100 Mats
03-252-163	No	Silicone	Step Base, Small Penetration Area	5 Mats
03-255-000	No	Silicone	Flat Base, Large Penetration Area	5
03-252-164	Yes	Silicone	Step Base, Small Penetration Area	5 Mats
03-252-162	Yes	Silicone	Flat Base, Large Penetration Area	5 Mats
03-252-170	No	Silicone, PTFE	Flat Base	5 Mats
03-252-171	Yes	Silicone, PTFE	Flat Base	5 Mats
01-213-156	No	Silicone Rubber	Flat Base, Large Penetration Area	50

Catalog No. 03-255-001 Supplier Thermo Scientific™ Supplier No. 60180M179

Description

Use SureSTART™ WebSeal™ 96-Well Plate Sealing Mats, 7 mm Diameter, Round, Performance Level 2 for high-throughput chromatography analysis. They are specification certified and conform to ANSI-SLAS 4-2004 standards.

Thermo Scientific™ SureSTART™ Level 2 WebSeal™ 7 mm Diameter Round 96-Well Plate Sealing Mats are designed to ensure high quality data with an uninterrupted workflow for high-throughput applications using GC and HPLC/UHPLC and are ideal for regulated labs that use water and polar solvents.

These 96-well plate sealing mats are specification certified to ensure autosampler compatible physical dimensions and conform to ANSI-SLAS 4-2004 standards.

Performance Level 2 Mats – for High-Throughput Applications 

• Ideal for non-regulated labs using water and polar solvents
• GC-MS and LC-MS tested
• Suitable for HPLC/UHPLC,QA/QC, and sensitive applications
• Ideal for verifying compound presence and quantification using detection techniques such as UV, DAD/PDA, RI, fluorescence, and ELSD
• Eliminates cross contamination of samples
• Resists coring and tearing
• Superior resealability after multiple injections
• WebSeal mats are manufactured from EVA (Ethylene-vinyl acetate) or pure silicone with or without a PTFE coating
• Silicone mats are dry heat autoclavable with excellent chemical compatibility, and can withstand low temperatures down to -80°C

Reduce the risk of mis-injection – To seal your plates correctly, it’s important to use the right mat

• Silicone Mats – Using a silicone mat with the correct profile reduces sample and solvent evaporation during your workflows, provides better consistency, and is ideal for long-term storage.
• Pre-slit Mats – Pre-slit mats are effective at reducing mis-injections and can be used again for confirmatory runs on the same plates without solvent loss. Their slits allow the use of pipette tips as well as multichannel pipettes.
• Adhesive Seals – Adhesive seals are effective as a one-time seal, but cannot be used for repeat runs. The exception is our three-layer re-sealing tape without glue at the cavity area.

Specification Certified  

• All Thermo Scientific SureSTART WebSeal mats are dimensionally verified 

Lot Traceable

• 100% traceable through the manufacturing process 
• Certificate of Conformance available by lot number upon request 

About Thermo Scientific WebSeal Well Plates & Mats

Thermo Scientific WebSeal plates and mats are available in three performance levels:

• Level 1 products are for everyday applications and are suitable for non-regulated labs using water and polar solvents
• Level 2 products are for high-throughput applications and are suitable for regulated labs using water and polar solvents
• Level 3 products are for high performance applications and are suitable for regulated labs using all solvent types including non-polar solvents

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Specifications

• Color: Clear
• Solvent Or Alcohol Resistant: Alcohol Resistant
• Material: EVA
• Sterility: Non-sterile
• No. of Wells: 96
• No. per Pack: 100 Mats
• Plug Diameter: 7 mm
• For Use With (Application): HPLC, UHPLC, GC
• Format: Domed Base
• Pre-slit: No
• Product Line: SureSTART WebSeal Level 2
• Adhesive: None
• Pierceable: Yes
• Temperature Range (Metric): -70°C to +65°C
• Well Shape: Round
• Plate Compatibility: WebSeal 96-well Round Well Plate

Frequently Asked Questions (FAQs)

Have the WebSeal mats been tested for evaporation?

Yes, all WebSeal mats that may be used with the WebSeal well plates have been subjected to evaporation tests which are either gravimetric or chromatographic. Gravimetric testing results show the retention of volatile solvents over periods of hours to days. The chromatographic evaluation shows the repeatability of low concentration standards over typical chromatographic analytical cycles.

What is the difference between the SureSTART Performance Level 1, Performance Level 2, and Performance Level 3 vials?

All glass vials found within the three different performance levels are made from Type 1 Class A Borosilicate glass. This type of glass has excellent chemical resistance properties that make it ideal for the containment or storage of acidic, neutral and alkaline samples/analytes. The vials also have high temperature resistance and are suitable for use at temperatures between -50 degrees C to 120 degrees C (although a rapid change in temperature of the products should be avoided).

The Performance Level 1 vials are designed to identify those which are suitable for simple routine, testing with limited budget. Performance Level 1 glass vials have a single wavy line.

For most analytical requirements, the Performance Level 2 products will be suitable for use with higher sensitivity methods while maintaining throughput. Performance Level 2 glass vials have two wavy lines.

For the most stringent analytical requirements, the Performance Level 3 products have advanced features which make them suitable for use with difficult samples in terms of chemistry and available sample volume. All Performance Level 3 products are made from 33-expansion glass and these vials have three wavy lines.

What happened to National, Chromacol, and other brands for chromatogenic workflow accessories?

Many of the products that were branded as National Scientific and Chromacol have been absorbed into the new Thermo Scientific SureSTART Collection, although there are still a few products that still exist within the marketplace.

For more information, please contact your local sales representative or complete the inquiries form at the bottom of the SureSTART Autosampler Vials and Caps page (https://www.thermofisher.com/us/en/home/industrial/chromatography/chromatography-consumables/autosampler-vials-caps-hplc-gc.html).

What is the SureSTART products portfolio for chromatographic workflows?

The SureSTART portfolio is a comprehensive list of vials, caps, inserts, well plates, mats and accessories that are suitable for use with chromatographic workflows. The SureSTART portfolio contains three different performance levels that have been quality checked to provide a robust and reliable product.

The SureSTART portfolio includes products that were previously found in our legacy brands such as National Scientific, Chromacol, and Sun-Sri.

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.