Invitrogen™ Wisteria Floribunda (Japanese Wisteria) Lectin (WFL), fluorescein (FITC)

Catalog No.	Quantity
L32481	2 mg

Catalog No. L32481 Supplier Invitrogen™ Supplier No. L32481

Description

Bright-green WFA-fluorophor conjugate

• Wisteria Floribunda Lectin (WFA, WFL), fluorescein (FITC), is a bright-green WFA-fluorophor conjugate. WFA is sourced from Wisteria floribunda (common name: Japanese wisteria), which is a species of flowering plant in the pea family Fabaceae, native to Japan. Due to the specificity of WFA, it has been used in many fields to label certain cells.
• In neurobiology, it has been used to detect perineuronal net. In the gastrointestinal tract, it stains the foveolar epithelium of normal gastric mucosa. This lectin has also been used as a marker of certain types of ovarian cancer.
• Very bright FITC conjugate detected with traditional FITC/GFP filter set, Ex/Em: 495/515 nm
• Applications: immunofluorescence, glycobiology
• Sugar specificity: N-acetylgalactosamine
• Inhibiting/eluting sugar: 200 mM N-acetylgalactosamine
• Blood group specify: non-specific
• Unconjugated lectin molecular weight: 116 KDa
• Immunofluorescence working concentration: 5–20 μg/mL
• WFA-FITC is supplied at a 2 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM Ca²⁺
• A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4°C, warm to 37°C and centrifuge before use.

Specifications

• Quantity: 2 mg
• Source: Wisteria floribunda
• Conjugate: Fluorescein
• Content And Storage: When stored at or below 2–8°C in the dark, the product is stable for 6 months after reciept.
  
  
• Label Type: Classic Dyes
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
• Form: Solution
• Protein Subtype: Lectins

For Research Use Only. Not for use in diagnostic procedures.