Gibco™ Wnt Surrogate-Fc Fusion Recombinant Protein

Description

Recombinant Protein

Gibco Wnt Surrogate-Fc Fusion Protein activates similar Wnt signaling pathways as most commercially available recombinant Wnt3a proteins or Wnt-conditioned media, while requiring a fraction of the amount of protein or hassle. Gibco Wnt Surrogate-Fc Fusion Protein was engineered with improved solubility, overcoming a major obstacle of working with Wnt3a and reducing the concentrations required to activate Wnt signaling in your model system. Gibco Wnt Surrogate-Fc Fusion Protein is comprised of the LRP-binding domain of DKK linked to a selective, high affinity binder of Frizzled receptors. Gibco Wnt Surrogate-Fc Fusion Protein is provided at a concentration of 2 mg/mL which is equivalent to 17.1 μM based on dimeric stoichiometry. Storage: Store undiluted Gibco Wnt Surrogate-Fc Fusion Protein at -80° for up to 1 year. After thawing, apportion into working aliquots and store at -20°C to -80°C for up to 2 months.

Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.

Specifications

• Accession Number: P56704
• Concentration: 2 mg/mL
• For Use With (Application): Functional Assay
• Formulation: 25 mM tris with 500 mM NaCl and no preservative; pH 8.2
• Gene ID (Entrez): 89780
• Name: Wnt Surrogate-Fc Fusion
• Quantity: 500 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: 1 EU/mL
• Gene Alias: MGC119418; MGC119419; MGC119420; Protein Wnt-3 A; vestigial tail; vt; wingless-related MMTV integration site 3 A; wingless-type MMTV integration site family member 3 A; wingless-type MMTV integration site family, member 3 like; wingless-type MMTV integration site family, member 3 A; Wnt 3 A; Wnt family member 3 A; wnt[a]; wnt3; wnt3 l; wnt3 like; Wnt3a; Wnt-3 A; WNT3A protein; wnt3l; Zwnt[a]
• Common Name: WNT3A
• Gene Symbol: WNT3A
• Biological Activity: Greater than 25 fold induction in Topflash assay activity (at a Wnt surrogate-Fc protein concentration between 0.03 - 5.0 nM (based on dimeric stoichiometry))
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Content And Storage: -20°Cor to +80°C if preferred
• Expression System: HEK293 cells
• Form: Liquid
• Purity or Quality Grade: >95%

Safety and Handling

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