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New England Biolabs, Inc. WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) - 100 rxn
Supplier: New England Biolabs, Inc. E1708S
The WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) is designed to provide a simple one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. This kit includes the WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution and a vial of LAMP Fluorescent Dye an intercalating dye provided at 50X concentration that can be used to track a LAMP reaction in real-time by fluorescence measurement. The inclusion of thermolabile UDG prevents carryover contamination where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions.
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