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Yeast Casitone Fatty Acids Agar with Carbohydrates (YCFAC) is an enriched nonselective media for the cultivation of most anaerobic bacteria and other fastidious microorganisms. YCFAC, when handled and incubated appropriately, has been shown to recover a wide range of difficult to culture organisms from gut microbiota samples. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.YCFAC is an enriched nonselective media used in the isolation and cultivation of a wide variety of bacteria found in the human gut, including Faecalibacterium prausnitzii. The basic nutritive components of this media come from yeast extract and pancreatic digest of casein. This basal medium is then enriched with various specific vitamins, sugars, and short chain fatty acids to ensure growth of the most fastidious gut microbes.