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Biotium Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit (TO and Live-or-Dye™ 568/583)
Supplier: Biotium 31064
The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583. Live-or-Dye™ 568/583 is a fixable red dye, designed for discrimination between live and dead cells during flow cytometry or microscopy. This dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. Thiazole Orange is a green fluorescent dye that localizes to the cytoplasm, with some nuclear accumulation. It is fixable, resulting in diffuse cytoplasmic staining in fixed cells. After staining yeast with this kit, both living and dead cells will be stained with green fluorescence (Ex/Em 512/533 nm). Only dead cells will be stained with red fluorescence (Ex/Em 568/583 nm), and may appear yellow when the two images are overlaid. This kit has sufficient reagent for 1000 assays.
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