Invitrogen™ Human YKL-40 ELISA Kit

Description

ELISA

The Human YKL-40 (CHI3L1) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human YKL-40.

Human cartilage glycoprotein 39 (GP-39), also known as YKL-40, is a glycoprotein secreted by articular chondrocytes, synoviocytes and macrophages. Serum and synovial fluid GP-39 levels are elevated in inflammatory diseases and correlate with the degree of joint destruction in rheumatoid arthritis. GP-39 is expressed in articular chondrocytes and synovial cells, as well as in liver, but is undetectable in muscle tissues, lung, pancreas, mononuclear cells and fibroblasts. GP-39 is a candidate autoantigen in rheumatoid arthritis and is important in the capacity of cells to respond to and cope with changes in their environment.

Specifications

• Accession Number: 39 kDa synovial protein; ASRT7; AW208766; Breast regression protein 39; Brp39; BRP39 protein; Cartilage glycoprotein 39; CGP39; CGP-39; Chi3l1; Chil1; chitinase 3 like 1; Chitinase 3 like protein 1 CGP 39; chitinase 3-like 1; chitinase 3-like 1 (cartilage glycoprotein-39); chitinase 3-like 1 protein; chitinase-3-like protein 1; chitinase-like 1; DKFZp686N19119; FLJ38139; Gp39; GP-39; hCGP39; HC-gp39; hCGP-39; HCGP-3 P; YKL40; YKL-40; YYL-40
• Assay Range: 78 to 5000 pg/mL
• Assay Sensitivity: 10.83 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1116
• Gene Symbol: CHI3L1
• Interassay CV: 7.2%
• Intraassay CV: 2.3
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 5 μL, Plasma 5 μL, Serum 5 μL
• Storage Requirements: 2°C to 8°C
• Target: CHI3L1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins