Z-LLNle-CHO (CAS No 133407-83-7) is a synthetic peptide aldehyde used in biomedical research particularly as a protease inhibitor This compound is derived from a combination of peptide chemistry and organic synthesis targeting proteolytic enzymes such as calpains Its mechanism of action involves binding to the active site of these enzymes inhibiting their proteolytic activity which is crucial in various cellular processes Z-LLNle-CHO exhibits in vitro activity in the nanomolar to low micromolar range making it effective in studies where precise inhibition of protease activity is required Typical applications include experiments with cultured cell models assessments in animal studies and high-throughput drug screening for potential therapeutic agents targeting protease-related pathways Experimental concentrations typically depend on the specific research design encompassing a range of conditions tailored to the study objectives