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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 21 Peptide

Catalog No. PV4535
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800 x 20 μL Assays
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PV4535 800 x 20 μL Assays
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Catalog No. PV4535 Supplier Thermo Scientific™ Supplier No. PV4535
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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This substrate is based upon CDC25C, containing Ser-216.

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Specifications

Product Line Z ́-LYTE
Assay Serine/Threonine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Content And Storage Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Excitation/Emission 405/465, 535 nm
Format 384-well Plate
For Use With (Application) FRET
Label or Dye Methylcoumarin
No. of Reactions 800 Reactions
Quantity 800 x 20 μL Assays
Readout End Point
Shipping Condition Dry Ice
Source Based on CDC25C Residues 207-225, Centered at Ser-216 (SRSGLYRSPSMPENLNRPRL)
Substrate Properties Peptide-Based Substrate
Target Entry MARK1, MARK2, BRSK1
Target Enzyme Serine/Threonine Kinases
Validated Kinases BRSK1, MARK1, MARK2
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For Research Use Only. Not for use in diagnostic procedures.

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