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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Tyrosine 7 Peptide

Catalog No. PV4305
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800 x 20 μL Assays
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PV4305 800 x 20 μL Assays
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Catalog No. PV4305 Supplier Thermo Scientific™ Supplier No. PV4305
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A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon LAT, containing Tyr-200.

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Specifications

Product Line Z ́-LYTE
Assay Tyrosine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Content And Storage Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. See COA for specific storage instructions Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Excitation/Emission 405/465, 535 nm
Format 384-well Plate
For Use With (Application) FRET
Label or Dye Methylcoumarin
No. of Reactions 800
Quantity 800 x 20 μL Assays
Readout End Point
References Biochem. J. 356 p 461-71 (2001)
Shipping Condition Dry Ice
Source Based on LAT, Residues 195 to 208, Centering Y-200 (ESIDDYVNVPESGES)
Substrate Properties Peptide-Based Substrate
Target Entry ZAP70
Target Enzyme Tyrosine Kinases
Validated Kinases ZAP70
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For Research Use Only. Not for use in diagnostic procedures.

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