Invitrogen™ Human ZAP-70, His Tag Recombinant Protein

Catalog No.	Quantity
P2782	20 μg

Catalog No. P2782 Supplier Invitrogen™ Supplier No. P2782

Description

Recombinant Protein

Protein Form: Full Length, Recombinant, Cytoplasmic Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate.

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Specifications

• Accession Number: P43403
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 25mM HEPES with 0.5mM DTT, 50% glycerol, 150mM NaCl and no preservative; pH 7.5
• Gene ID (Entrez): 7535
• Molecular Weight (g/mol): 70.1 kDa
• Name: Human ZAP-70, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 20 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
• Common Name: Zap-70
• Gene Symbol: ZAP70
• Kinase Family: SYK Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Full length
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic
• Kinase Group: Tyrosine Kinases
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate
• Target Entry: ZAP70
• Target Gene: zeta-chain (TCR) associated protein kinase
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Full Length, Recombinant, Cytoplasmic
• Form: Liquid
• Protein Subtype: Cytoplasmic Tyrosine Kinases
• Research Category: Signal Transduction