Invitrogen™ Zenon™ pHrodo™ iFL IgG Labeling Reagents

Catalog No.	Quantity	Species	Label or Dye	Color
Z25614	1.2 mL	Human	pHrodo iFL Red	Red
Z25618	250 μL	Human	pHrodo Deep Red	Deep Red
Z25622	250 μL	Mouse	pHrodo Deep Red	Deep Red
Z25612	250 μL	Human	pHrodo iFL Red	Red
Z25611	250 μL	Human	pHrodo iFL Green	Green
Z25610	250 μL	Mouse	pHrodo iFL Red	Red
Z25609	250 μL	Mouse	pHrodo iFL Green	Green
Z25623	1.2 mL	Human	pHrodo Deep Red	Deep Red
Z25613	1.2 mL	Human	pHrodo iFL Green	Green
Z25624	1.2 mL	Mouse	pHrodo Deep Red	Deep Red
Z25626	1.2 mL	Mouse	pHrodo Red	Red
Z25625	1.2 mL	Mouse	pHrodo Green	Green

Catalog No. Z25614 Supplier Invitrogen™ Supplier No. Z25614

Description

Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex.

Streamline antibody-drug conjugate screening campaigns with Invitrogen Zenon pHrodo IgG Labeling Reagents to screen for antibody internalization. These reagents provide rapid, scalable antibody labeling and give a bright fluorescence-based indication of antibody uptake and trafficking in target cells when analyzed by flow cytometry, fluorescence microscopy, or high-content screening.

Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex. Formation of the Fab–antibody complex occurs in fewer than 5 minutes. Because the pHrodo™ iFL dyes dramatically increase in fluorescence as the pH of their surroundings becomes more acidic, the antibodies coupled with the Zenon™ pHrodo™ iFL IgG Labeling Reagents provide an excellent measure of endocytic activity based on the acidification of the labeled antibodies as they are ingested by the cell.

The Zenon™ pHrodo™ iFL IgG Labeling Reagents contain sufficient material to label one to four 96-well plates, depending on the Zenon™ labeling reagent concentration used. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Other advantages of the pH-sensitive Zenon™ pHrodo™ iFL IgG Labeling Reagents include:

• Simplicity—No reagent purification is required
• Accuracy—No wash steps or quenchers are needed, minimizing cross-reactivity or background
• Speed—Labeled antibodies are typically ready to use in 10 minutes
• Specificity—Dyes are designed for the specific detection of phagocytosis and endocytosis

Preserve primary antibody function and affinities

Reactive dye labeling of primary antibodies can have unpredictable and undesirable outcomes. One of these is reduced binding affinity due to label addition in the binding pocket. The Zenon™ antibody labeling approach, which is targeted to the Fc tail, resolves this potential issue.

Try custom antibody conjugates

If the antibody conjugate you need is not available as one of our stocked products, we will prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Order Info

Store in refrigerator at 2°C to 8°C.

Specifications

• Description: pHrodo™ iFL Red, 1.2 mL
• Product Type: Labeling Reagent
• Quantity: 1.2 mL
• Content And Storage: Store in refrigerator (2°C to 8°C) and protect from light
• Excitation/Emission: 560/585 nm
• Product Line: Zenon
• Label or Dye: pHrodo iFL Red
• Color: Red
• Labeling Target: IgG
• Species: Human
• Label Type: pHrodo™ (pH sensitive)

For Research Use Only. Not for use in diagnostic procedures.