ZeptoMetrix OXItek Glutathione Reductase Assay Kit

Description

Includes

120mL of GR-assay buffer, 4 vials of GR-reagent 1, 4 vials of GR-reagent 2, 25mL of GR-reagent 3, 1 vial of QC material.

Provides an indirect and highly reproducible method of quantifying the activity of total cellular glutathione reductase

Glutathione reductase (GR, EC No. 1.6.4.2) is a flavoprotein that is required for the conversion of oxidized glutathione (GSSG) to reduced glutathione (GSH). At the same time, it oxidizes nicotinamide adenine dinucleotide phosphate (NADPH). This universally
present enzyme is essential for the maintenance of reduced glutathione (GSH) levels in vivo. Reduced glutathione plays an important role in oxidoreduction processes and detoxification of H₂O₂ and organic peroxides, which are substances produced in large quantities during inflammatory processes in living cells. Glutathione reductase therefore plays a major role in glutathione peroxidase (GPx) and glutathione stransferase (GST) reactions as an adjunct in the control of peroxides and free radicals.When levels of catalase, another universally present antioxidant enzyme, are decreased, the glutathione dependant enzymes become activated. A deficiency of glutathione reductase is characterized by hemolysis owing to the increased sensitivity of erythrocyte (RBC) membranes to H₂O₂ that lead to osmotic fragility. This reaction is thus required for the stability and integrity of red cells.

Specifications

• Certifications/Compliance: ISO 13485
• Product Type: OXItek Glutathione Reductase assay Kit
• Sufficient For: 100 Tests
• For Use With (Application): For quantifying the activity of to tal cellular Glutathione Reductase
• Kit Contents: GPx reagent 2 4 vial/kit, GPx-assay buffer 120 mL/kit, GPx-reagent 1 4 vial/kit, Gpx-reagent 3 25 mL/kit, QC material 1 vial/kit
• Quantity: 100 Tests
• Storage Requirements: 2°C to 4°C

Research purposes only. Not for in vitro diagnostic use.