The Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set is for the quantitative determination of Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E).The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) coated on a 96-well plate. Standards and samples are added to the wells, and any Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) present in the sample. To end the