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AdipoGen Zin3 AM (500 µg)
Supplier: AdipoGen CDXF0019C500
Chemical. CAS: 562102-46-9. Formula: C46H44F2N2O20. MW: 982.85. Synthetic. Zin3 AM is a cell-permeable Zn2+-selective indicator (Absorption=456nm), that once it enters the cell is hydrolized by esterases to convert the bright fluorescent Zin3 (Ex/Em: ~494/516nm). Zin3 is suitable for detection of Zn2+ concentrations in the 1–100 nM range and it has shown to be very Zn2+-sensitive and Zn2+-specific. The cell permeant AM-ester is useful for detecting low intracellular Zn2+ levels and small concentration changes. Zin3 exhibits high Zn2+-binding affinity that is unperturbed by Ca2+ concentrations up to at least 1 µM (Kd (Zn2+) (in buffer): ~15nM). In addition, Zin3 exhibits a >50-fold increase in fluorescence in response to saturating levels of Zn2+. This dye is usedpredominantly for imaging applications, but has also used in cell-based microplate assays and flow cytometry protocols. The product is typically loaded into cells by adding the dissolved indicator to medium containing cells.
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