AdipoGen ZnAF-1F

Supplier:  AdipoGen CDXZ0009M005

Chemical. CAS 443302-08-7. Formula C34H26F2N4O5. MW 608.59. Synthetic. ZnAF-1F and ZnAF-2F do not fluoresce in the presence of other biologically important cations such as Ca2 and Mg2, and are insensitive to change of pH. The complexes with Zn2 of previously developed ZnAFs, ZnAF-1, and ZnAF-2 decrease in fluorescence intensity below pH 7.0 owing to protonation of the phenolic hydroxyl group of fluorescein, whose pKa value is 6.2. On the other hand, the Zn2 complexes of ZnAF-1F and ZnAF-2F emit stable fluorescence under neutral and slightly acidic conditions because the pKa values are shifted to 4.9 by substitution of electron-withdrawing fluorine at the ortho position of the phenolic hydroxyl group. Spectral data lambdaex 492nm lambdaem 517nm in PBS.

Catalog No. 50-255-9881