Used in Brown and Brenn Method for gram positive and gram negative Bacteria.
Also see the Taylor Modification of the Brown and Brenn +/- technique noted for the varying differentiation available. Over-differentiation in the B&B sep 6 is a problem with some sections; run the control slides at varying rates to determine the amount for the specific organism.
References are Brown J.H. and Brenn, L. Bull. Johns Hopkins Hosp., 48:69 (1931) and AFIP Manual of Histologic Staining Techniques: 3rd. ed., ed. G. Luna; New York: McGraw-Hill Publications, c. 1968, p. 222