Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Quality Biological Inc PBS pH 7.2, 10 Liters
PBS (phosphate buffered saline) is a balanced salt solution ¿ Used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents ¿ Endotoxin and Mycoplasma tested ¿ Also useful in immunological applications, such as Western Blotting ¿ Storage Condition: 15¿C ¿ 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.1 -7.3 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Apexbio Technology LLC Annexin V-HF647/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes PI is a dye which specifically binds double-stranded DNA and emits red fluorescence under normal circumstances it cannot penetrate intact cell membranes However in late apoptotic and dead cells PI penetrates the cellular membrane staining the nuclei Dual staining with Annexin V-HF647 and PI enables clear differentiation between early apoptotic late apoptotic and dead cells
Teknova 1X PBS with 0.05% Tween 20, 0.1% BSA. 1000mL, Sterile. 8 Pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 1X PBS-MK with Sodium Chloride, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
TRIBIOSCIENCE INC Inoue Transformation Buffer, 500ML
Inoue Transformation Buffer is used for the preparation of high-efficient competent cells. This product is based on the Inoue method for the preparation and transformation of E. Coli component cells.
Teknova 1X PBS Solution, pH 7.4. 100mL, Sterile. 10 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
MSC Value Collection Magnifier, 20x, Dual Lens, Round, Metal
The Value Collection magnifier is a durable tool designed for detailed inspection. It features a high 20x magnification, a dual-lens system, and a robust metal construction, making it suitable for various precision tasks.
- 20x maximum magnification
- Dual lens system
- Round lens shape
- Metal construction
Med Vet International Doxycycline Hyclate Tablets (Oracea), 100mg, 50ct
VET LICENSE NEEDS TO BE PROVIDED IN 3-4 BUSINESS DAYS OR ORDER WILL BE CANCELLED
Doxycycline hyclate tablets is a generic version of Oracea. It belongs to a class of antibiotics known as tetracyclines. It should be stored at room temperature between 59-86 degrees F and away from light and moisture.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 500ml
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.