Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific TE Buffer, 1X, 1 L
TE Buffer, 1X, is a ready-to-use buffered solution formulated for a wide range of molecular biology applications. This 1L bottle is ideal for preparing buffers for routine experiments, including nucleic acid extraction, PCR, and gel electrophoresis. The TE Buffer maintains a stable pH and osmolarity, which is essential for protecting nucleic acids and ensuring reliable results during processing. The 1X concentration is optimal for immediate use in applications that require a stable buffering system to maintain the integrity of RNA, DNA, and other biochemical compounds. It is commonly used in laboratories for standard molecular protocols and can be stored for extended periods without degradation.
![Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 100 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661046.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 100 Tablets per Bottle
PBS [Phosphate Buffered Saline] 100 mL Tablets are solid tablets that dissolve easily to prepare 100 mL of phosphate-buffered saline. These tablets provide a convenient and efficient way to prepare PBS solutions, eliminating the need for weighing and measuring individual components. They are ideal for laboratory applications that require consistent and reliable buffer preparation, including cell culture, microbiological testing, and molecular biology procedures. The tablets dissolve quickly in water, ensuring that the resulting buffer maintains the correct pH and osmolarity for a wide range of applications. Each bottle contains 100 tablets, making it a cost-effective option for labs that require frequent PBS preparation.
![Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502387606.png-250.jpg)
Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.
1.54M Sodium Chloride,55mM Sodium Phosphate Dibasic,12mM Potassium Phosphate Monobasic
Kd Medical Inc PBS 1X, PH 7.4 W/SOD AZIDE,20L
Phosphate Buffered Saline is used as a wash buffer in several lab applications. Contains sodium azide FACS washing grade, pH 7.4. One 20 liter cubitainer.
Research Products International Corp PBS, 10X Powder Concentrate, 10 L
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.
FUJIFILM BIOSCIENCES INC Iscoves Modified Dulbeccos Med
Iscoves Modified Dulbeccos Medium IMDM is a modification of Dulbeccos Basal Medium Eagle BME that uses high glucose and pyruvate additional amino acids 25mM HEPES selenium and other components IMDM is designed for serumfree growth of hemopoietic cells in culture and supports the growth ofLymphocytes and a variety of other hybrid cells
Alkali Scientific CellPro™ PBS Buffer, 1X pH 7.2, 100mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 500 Tablets per Bottle
PBS [Phosphate Buffered Saline] 100 mL Tablets are designed to make buffer preparation simple and efficient. Each tablet dissolves easily in water to create 100 mL of phosphate-buffered saline. With 500 tablets per bottle, these tablets are perfect for laboratories with high-volume buffer preparation needs. The ease of use and consistency of the PBS solution make these tablets ideal for various applications, including cell culture, microbiological analysis, and molecular biology experiments. The tablets provide a reliable way to ensure that each PBS solution is accurately prepared with the correct pH and osmolarity, minimizing the potential for human error in buffer formulation.
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
Alkali Scientific CellPro™ PBS, 10X, pH 7.4, 500mLCellPro™ PBS, 10X, pH 7.4, 500mL
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This modification has been shown to improve the stability of the medium.
![Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661850.jpg-250.jpg)
Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters
DPBS 1X (Dulbecco's Phosphate Buffered Saline) is a sterile saline solution used in biological research and clinical laboratories. The 500mL bottle is a convenient size for smaller laboratories or specific experiments that require moderate volumes of buffer solution. DPBS is widely used for washing cells, preparing tissues, and diluting reagents in cell culture, molecular biology, and microbiology applications. The balanced composition of salts and phosphate ensures that the solution maintains osmotic pressure and pH stability, making it essential for maintaining cell viability and supporting biological assays that require precise environmental conditions.
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 5mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes