Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Pickering Laboratories Inc LITHIUM ELUANT 2.75 4X950ML
Lithium Eluant, pH 2.75, case of 4 (950 mL/bottle) Amino Acid Analysis.
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solution1L
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 100 G
MES (2-Morpholinoethanesulphonic acid) hemisodium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- It is a zwitterionic buffer and provides effective buffering capacity around pH 6.15.
- The pKa value changes with temperature, requiring consideration for its use at different temperatures.
- It shows good buffering properties in biological experiments.
- It exhibits poor affinity with metal ions, allowing its use in studies involving metal ions.
Medchemexpress LLC Mes (2-morpholinoethanesulphonic acid) | 4432-31-9 | 99.85% | 1 ML
MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer designed for various biochemistry and molecular biology applications. It functions effectively within a pH range of 5.5-7.0 and is widely recognized as a Good's buffer, suitable for experiments including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity from pH 5.5-7.0
- Ideal for cell culture, enzyme activity determination, electrophoresis, and protein studies
- Solid, white to off-white appearance
- Soluble in water and DMSO
Medchemexpress LLC Heppso | 68399-78-0 | 99.89% | 100 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and possesses a pKa of 7.84 at a 2.0 mM buffer concentration. It is intended for research use only and is not sold to patients.
- Working pH range of 7.1-8.5.
- Exhibits a relatively high ability to bind copper(II).
- Possesses a pKa of 7.84 at a 2.0 mM buffer concentration.
- For research use only.
- Molecular weight: 268.33.
- Formula: C9H20N2O5S.
- Appearance: Solid.
- Color: White to off-white.
![Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000409966-logo.png-250.jpg){kind=logo}
Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G
TAPS is an aminosulfonate-based lysozyme stabilizer and connexin channel inhibitor. It helps maintain the native structure of lysozyme in aqueous solution at pH 7.0 and protects it from heat-induced denaturation. TAPS is also considered a critical compound for investigating the structure and function of connexin channels.
- Maintains native structure of lysozyme in aqueous solution at pH 7.0
- Protects lysozyme from heat-induced denaturation
- Directly inhibits heteromeric connexin 32/connexin 26 channels (Cx32/Cx26)
- Reduces connexin channel-mediated solute exchange in a recombinant liposome system
- Critical compound for investigating connexin channel structure and function
Boston Bioproducts Inc HBS-P+ Buffer (10X, pH 7.4) - 4x500ml
HBS-P Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-EP+ Buffer (1X, pH 7.4) - 4x500ml
HBS-EP Plus Buffer (1X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Molecular Biology Grade Water - 1L
Molecular Biology Grade Water - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Saponin Cell Permeating Solution (0.5% w/v in PBS) - 4x100ml
Saponin Cell Permeating Solution (0 5% w/v in PBS) - 4x100ml (For Research Use and Further Manufacturing Only)
eMolecules 1-(3-Ethyl-4-(hydroxymethyl)phenyl)ethanone | 1378888-43-7 | MFCD18910891 | 1g
Ambeed | 1-(3-Ethyl-4-(hydroxymethyl)phenyl)ethanone | 1g | 600847859 | A790327 | | 1378888-43-7 | MFCD18910891 | 178.231 | C11H14O2
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Boston Bioproducts Inc Potassium Phosphate Buffer (0.2 M, pH 7.4) - 1L
Potassium Phosphate Buffer (0 2 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)