Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPBS >=99% (titration) | 161308-36-7 | MFCD00677124 | 100G
HEPBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 266.36 | 161308-36-7 | MFCD00677124 | 100G
National Diagnostics SEQUAGEL BUFFER 200 ML
SequaGel Buffer, System consists of SequaGel Concentrate, SequaGel Diluent, and SequaGel Buffer for casting 19:1 denaturing DNA gels of a varying percentage, 200 ML
Teknova 50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
![Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B78000-1000.0.JPG-250.jpg)
Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kilogram
- Substance Name: 1,3-Bis (TRIS(hydroxymethyl)methylamino)propane
- CAS Number: 64431-96-5
- Molecular Formula: C11H26N2O6
- Molecular Weight: 282.3
Kd Medical Inc TRIS BUFF SALINE,10X PH 7.6 1L
Commonly used buffer used as a wash buffer in multiple laboratory applications. 10X concentrated TBS is ready to be diluted to a 1X working solution. pH 7.6, one 1 Liter bottle.
Teknova 100mM Tris-HCl, pH 8.2. 1000mL, Sterile 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.5) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 8 5) - 2x1L (For Research Use and Further Manufacturing Only)