Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ Tris-HCL Buffer, 5M pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Crescent Chemical Co Inc EDTA ANALYTICAL GRADE
Ethylenediamine tetraacetic acid analytical grade Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 11278.02 CAS 60-00-4
Biotang Inc TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
Bioworld PEB- Protein Extraction Buffer (5x), 250 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
Kd Medical Inc TRIS HCL, 1M, PH 7.5, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 7.5. One 500ml bottle.
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-100.0.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 100 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
![Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40020-100.0.JPG-250.jpg)
Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 100 Grams
CHES, also known as 2-(N-Cyclohexylamino) ethanesulfonic acid, is a chemical compound that plays a significant role in life science research, particularly in molecular biology and protein research.
CHES is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 8.6 to 10.0, making it suitable for applications requiring higher alkaline pH conditions.
CHES is also commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability in alkaline conditions make it valuable in these applications.
One of the significant advantages of CHES is its low ionic strength, which minimizes interference with biological processes and enzymatic activities.
Chem-Impex International, Inc. Piperazine-N,N'-bis(2-ethanesulfonic acid) | 5625-37-6 | MFCD00006159 | 100G
Piperazine-N,N'-bis(2-ethanesulfonic acid), 5625-37-6, MFCD00006159, 100G
Medchemexpress LLC Mes (2-morpholinoethanesulphonic acid) | 4432-31-9 | 99.85% | 1 ML
MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer designed for various biochemistry and molecular biology applications. It functions effectively within a pH range of 5.5-7.0 and is widely recognized as a Good's buffer, suitable for experiments including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity from pH 5.5-7.0
- Ideal for cell culture, enzyme activity determination, electrophoresis, and protein studies
- Solid, white to off-white appearance
- Soluble in water and DMSO
Medchemexpress LLC Heppso | 68399-78-0 | 99.89% | 100 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and possesses a pKa of 7.84 at a 2.0 mM buffer concentration. It is intended for research use only and is not sold to patients.
- Working pH range of 7.1-8.5.
- Exhibits a relatively high ability to bind copper(II).
- Possesses a pKa of 7.84 at a 2.0 mM buffer concentration.
- For research use only.
- Molecular weight: 268.33.
- Formula: C9H20N2O5S.
- Appearance: Solid.
- Color: White to off-white.
Medchemexpress LLC Ada disodium | 41689-31-0 | 98.0% | 10 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, especially in the preparation of enzyme buffers. It is for research use only.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Used in the preparation of enzyme buffers