Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-50.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 50 Grams
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Bioworld Tris Buffered Saline Packs, 40 PK(s)
Tris Bufferd Saline Packs An ideal all-purpose binding buffer.Each pack yields 500 ml of 25 mM Tris, 0.15 M NaCl, pH 7.2 when dissolved in 500 ml deionized water (total 5 liters). Quantity: 10 packs
Research Products International Corp TRIS-Tricine-SDS Running Buffer 10X Solution, 500 ML
10X Formulation:
Tricine: 1 M
SDS (pH 8.3): 1%
Alkali Scientific CellPro™ Tris HCl Buffer, 0.5M, pH 8.5, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Bioworld TT Buffer, Ready-Pack (10X), 2 L
Tris-Tricine BufferTris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more conventionally used buffers. TT Buffer allows for optimal resolution of low molecular weight proteins and elimantes the glycine front often found in gel utilizing TG-SDS and other such beffers. This improved running buffer allows for resolution of peptides as low as 5kD. TT buffer is also excellent for protein electrophoresis where resolved products will be transferred to a nylon membrane. Unlike glycine, tricine will not interfere with membrane protein sequencing. The 10X pack just requires the addition of distilled H2O to make 1.0 Liter of 10X liquid. A simple 10-fold dilution with water provides a 1X solution, that makes an excellent running buffer for most applications. Technical Information:Ultra PureGradeStorage: RTHazard: Irritant
Teknova QB Citrate Buffer, pH 3.0. 1000mL, Sterile.
Sodium citrate solution is a commonly used reagent in life science. It has many uses, such as making media for the growth of microorganisms, lysing cells, and precipitating nucleic acids.
Sigma Aldrich Fine Chemicals Biosciences Tris-EDTA Buffer | CAS No.:Not specified| MDL No.: MFCD00236359 | 1 L
Tris-EDTA buffer | Purity: 100% | M. W.: Not applicable | CAS No.: Not Specified| MDL No.: MFCD00236359 | 1L
Teknova 2.5M Tris-HCl pH 7.5. Endotox
2.5M Tris-HCl, pH 7.5. Endotoxin Tested. 1000mL, Sterile. 6 pack.
Biotang Inc Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml
Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml.
IBI SCIENTIFIC 3P (EDTA) SOLUTION, 100 mL
IBI 0.5MEDTA solution (pH 8.0)is ideal for biochemistry and molecular biology applications requiring a chelator of divalent metal ions. Ethylenediamine tetraacetic acid solution can be used to elucidate the role of antigen retrieval in immunostaining of ethanol fixed smears.
Bioworld Bis-Tris, 100 g
Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane A common biological bufferAn inert, zwitterionic buffer that exhibits only a very small shift in dissociation constantSpecifications:Ultra Pure GradepKa (25°C): 6.5+/-0.2Appearance: SolidBoiling Point : 466.18 °C at 760 mmHg (Predicted)Density : 1.35 g/cm3 (Predicted)Melting Point: 102-105 °CPH Value: 5.8-7.2