Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp NP-40 Lysis High Salt Buffer Solution, 500 ML
1X buffer used for extracting proteins from cells.
![Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40020-100.0.JPG-250.jpg)
Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 100 Grams
CHES, also known as 2-(N-Cyclohexylamino) ethanesulfonic acid, is a chemical compound that plays a significant role in life science research, particularly in molecular biology and protein research.
CHES is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 8.6 to 10.0, making it suitable for applications requiring higher alkaline pH conditions.
CHES is also commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability in alkaline conditions make it valuable in these applications.
One of the significant advantages of CHES is its low ionic strength, which minimizes interference with biological processes and enzymatic activities.
Teknova DNA Suspension Buffer, pH 8.0. DNase/RNase Tested, PCR Grade. 1mL Tubes, 50-Pack, Sterile.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Teknova 1X PBS, 500mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Alkali Scientific CellPro™ Tris Buffered Saline 10X, pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific QuickSilver TBS Buffer Powder, 100 Pouches
QuickSilver™ Buffer Powder packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, and easy to use. Convenient premeasured pouches make 1L of 1X buffer. There is no pH adjusent needed and they offer a long shelf life. They come supplied with preprinterd bottle labels for easy identification. 100 pouches per pack.
Advanced Biomatrix Phosphate Buffered Saline 10X PBS 100 mL
Phosphate Buffered Saline (PBS) 10X is formulated and prepared to use in conjunction with other products provided by Advanced BioMatrix such as Type I collagen for forming a 3D hydrogel. PBS 10X is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
Boston Bioproducts Inc PIPES Buffer (1 M, pH 6.8) - 500ml
PIPES Buffer (1 M pH 6 8) - 500ml (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 100g
BICINE | Purity: 99% | Mol Wt: 163.17 | 150-25-4 | MFCD00004295 | 100g
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 1KG
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 1KG
Sigma Aldrich Fine Chemicals Biosciences Piperazine BioUltra, anhydrous, >=99.0% (T) | 110-85-0 | MFCD00005953 | 25G
Piperazine BioUltra, anhydrous, >=99.0% (T) | Purity: >=99.0% (T) | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 25G
Boston Bioproducts Inc Bicine Buffer (0.5 M, pH 8.0) - 250ml
Bicine Buffer (0 5 M pH 8 0) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 1L
Tris-HCl Buffer (1 M pH 7 6) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Potassium Phosphate Buffer (0.2 M, pH 7.4) - 1L
Potassium Phosphate Buffer (0 2 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)