Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Kd Medical Inc TRIS HCL 1M PH 8.O 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 8.0. One 500ml bottle.
Crescent Chemical Co Inc TE BUFFER (100X) PH 8.0
TE Buffer (100X) pH 8.0 DNase/RNase not detected. 100 x concentrated aqueous solution. Size - 100ML Storage Conditions - +15 °C TO +30 °C Catalog Number - 39799.01
Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.
Sigma Aldrich Fine Chemicals Biosciences Piperazine | 110-85-0 | MFCD00005953 | 100 g
Piperazine | Purity: ≥99.0% | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 100 g
