Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp TRIS-Tricine-SDS Running Buffer 10X Solution, 1 Liter
10X Formulation:
Tricine: 1 M
SDS (pH 8.3): 1%
Kd Medical Inc TRIS-HCL, 1M, PH 7.4, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 7.4. One 500ml bottle.
Electron Microscopy Sciences Pipes Buffer Solution 0.3m, pH 5.5-6.0 500 ML
PIPES is a hydrogen-ion organic-based buffer, used in the preparation of a variety of mammalian tissues for electron microscopy. It produces excellent results in the preservation of ultrastructural details, especially when long fixation is required. This buffer does not appear to contribute extraneous anions or cations to the tissue. Aqueous PIPES solution is adjusted by 0.1N Sodium Hydroxide to pH 5.5-6.0.
pH: 5.5-6.0
Electron Microscopy Sciences D-PBS, Formula III Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Electron Microscopy Sciences Tris (Hydroxymethyl) Aminomethane Free Base, Reagent 100 GR
CAS #77-86-1 C₄H₁₁NO₃ F.W. 121.14
Assay: >99.9%
White crystalline powder. A 40% (w/v) aqueous solution. Hydroxymethyl Aminomethane.
Grade: Reagent
Teknova 20X PBS Solution, pH 7.2 (at 1X). 10L, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Pyridoxal which is an aldehyde has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.
Sigma Aldrich Fine Chemicals Biosciences PIPES BioPerformance Certi1KG
PIPES BioPerformance Certi1KG
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 titration25KG
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Research Products International Corp HEPES Buffered Saline Solution 2X, 500 Milliliters
2X Formulation:
KCI: 10 mM
Na2HPO4: 1.5 mM
Dextrose: 12 mM
HEPES: 50 mM
pH: 7.0