Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
National Diagnostics PROTOGEL STACKING BUFFER 200 M
ProtoGel Stacking Buffer, 4X Laemmli Tris-HCl stacking gel buffer for clear, reproducible SDS-PAGE, 200 ML
Research Products International Corp TRIS BUFFERED SALINE, 10X SOLUTION, 4 LITER
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml.
eMolecules tert-Butyl 4-(hydroxymethyl)benzoate | 143726-85-6 | MFCD04973449 | 1g
Ambeed | tert-Butyl 4-(hydroxymethyl)benzoate | 1g | 592282779 | A321260 | | 143726-85-6 | MFCD04973449 | 208.257 | C12H16O3
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Biocare Medical, LLC Tris Buffer Saline (TBS) Plus, 10X
For laboratory use and procedures requiring a high quality Tris buffer saline. TBS is sodium azide and thimerosal free and provides superior pH stability. It can also be used for manual immunohistochemistry applications or on an automated staining system with the addition of surfactant.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | Purity: >=99.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG
CHEMSCIENCE Brass "A" for Sn, Pb, Cu, Zn
• This product is supplied in five lot of 500 g each of known concentration ( % concentration availiable upon request.)
• Detailed purity information, along with the Safety Data Sheet (SDS) and Technical Data Sheet (TDS), will be provided upon purchase.
Quality Biological Inc Tris HCl (2 M) pH 8.8 500mL
Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Use the Tris-HCl 0.5 M (pH 6.8) buffer for stacking gels. Use Tris-HCl 1.5 M (pH 8.8) buffer for resolving gels. Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.Manufactured in an ISO 90012015 certified facility.
Teknova 50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
Quality Biological Inc Tris HCl (1 M), pH 7.5, PK 4 X 100 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ These solutions of Tris Hydrochloride provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). ¿ Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ pH Range: 7.5 ¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Alkali Scientific CellPro™ Tris HCL, 1M pH 6.8, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.