Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Zymo Research Corporation ZymoPURE™ Binding Buffer (150 ml)
ZymoPURE™ Binding Buffer (150 ml). Assists in binding nucleic acid to ZymoPURE™ columns for purification.
Bioworld BES-Buffered Saline (2X), 250 mL
Product DescriptionBES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) saline buffer has a concentration of 2X and a pH of 7.1 at 25°C. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test. This saline buffer contains 50 mM BES, 1.5 mM Na2HPO4, and 280 mM NaCl.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Apexbio Technology LLC AO/PI Double Staining Kit 100T
The AO/PI Double Staining Kit is a reagent kit enabling rapid detection of cell viability This kit provides two nuclear dyes Acridine Orange (AO) and Propidium Iodide (PI) AO can penetrate intact cell membranes and stain viable cells green During apoptosis chromosomes become condensed or fragmented into differently sized segments In this scenario AO stains apoptotic cells densely with yellow-green fluorescence or as yellow-green fragments The other nuclear dye PI cannot pass through intact cell membranes therefore it does not stain viable or apoptotic cells in red but stains cells with compromised membranes (e g necrotic cells) When using these two dyes in combination viable cells exhibit green fluorescence apoptotic cells show dense varied-size yellow-green or orange fluorescence and necrotic cells exhibit strong red fluorescence
Apexbio Technology LLC Live-Dead Cell Staining Kit I (Calcein AM/PI) 100T
Calcein AM/PI Live-Dead Cell Staining Kit is a reagent kit used to distinguish between viable mammalian cells with esterase activity and dead cells The kit contains two probes Calcein AM and Propidium Iodide (PI) Calcein AM is a probe that stains live cells it is a derivative of Calcein with an added AM moiety which makes it more hydrophobic and allows better cell permeability Calcein AM itself is non-fluorescent but once it enters live cells it is hydrolyzed by intracellular esterases into Calcein which can emit strong green fluorescence PI is a probe for dead cells which enters only cells with compromised membranes staining them red The fluorescence intensities of these two probes reflect cell viability and toxicity The kit is easy to use 30 minutes of staining incubation After staining results can be detected using fluorescence microscopy fluorescence microplate readers or flow cytometry This kit is unsuitable for bacteria or fungi
Growcells PBS 1X 10L EA
Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 8g, KCl 0.2g, Na2HPO4 1.44g, KH2PO4 0.24g
Research Products International Corp TE Buffer 20X Solution pH 8.0, 250 Milliliters
Formulation:
EDTA: 20 mM
Quality Biological Inc Tris HCl (1 M), pH 7.5, PK 4 X 100 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ These solutions of Tris Hydrochloride provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). ¿ Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ pH Range: 7.5 ¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Mitegen Tris/ HCl, 1M, pH7.0, 250 ml, CAS # 77-86-1
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Chem-Impex International, Inc. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | 7365-45-9 | MFCD00006158 | 1KG
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, 7365-45-9, MFCD00006158, 1KG
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-100.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
Research Products International Corp PIPES SODIUM SALT, 1 KILOGRAM
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Research Products International Corp TRIS-BORATE-EDTA, 10X SOLUTION, 1 LITER
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
![Research Products International Corp TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60015-1000.03.JPG-250.jpg)
Research Products International Corp TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter
TAE 50X Buffer is a concentrated solution of Tris, acetic acid, and EDTA used in nucleic acid electrophoresis. When diluted, it maintains pH (~8.3) and low ionic strength, supporting clear DNA/RNA separation. EDTA chelates metal ions, helping prevent nucleic acid degradation during electrophoretic analysis in molecular biology workflows.
Substance Name: TRIS Acetate EDTA 50X Solution
Solution contains:
Acetic Acid: 1 M
EDTA, Disodium Salt Dihydrate: 50 mM