Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
STEMCELL Technologies EasySep™ Red Blood Cell Lysis Buffer, Size: 100 mL
EasySep™ Red Blood Cell Lysis Buffer is for use as directed with EasySep™ or RoboSep™ Whole Blood kits for the lysis of red blood cells prior to separation from whole blood.
Chem-Impex International, Inc. Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid, 60-00-4, MFCD00003541, 1KG
Medchemexpress LLC Nvp-TAE 684 | 761439-42-3 | MFCD11977634 | 99.7% | 614.20 | C30H40ClN7O3S | 1 ML
NVP-TAE 684 is a potent, selective small-molecule inhibitor of anaplastic lymphoma kinase (ALK) used in preclinical research to block the growth of ALCL-derived and ALK-dependent cell lines (reported IC50 2-10 nM). It is provided as both solid material and as a ready-to-use 10 mM solution in DMSO, and is characterized for purity suitable for in vitro studies.
- Potent ALK inhibition with low nanomolar IC50 values.
- Selective activity against ALK-dependent cell lines.
- Available as a 10 mM solution in DMSO for ready-to-use experiments.
- High purity (99.7%) suitable for research applications.
- Molecular weight 614.20 Da and formula C30H40ClN7O3S.
- Offered in multiple solid pack sizes for flexible dosing and storage.
- Documentation available including data sheet and SDS.
Bioworld TAE Buffer 50X, Liquid Concentrate 1L
TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended. Appearance Form : Liquid, Appearance Color : Clear. 1L
Bioworld Tris-Glycine-SDS (TGS) Running Buffer (10X), 1 L
Product DescriptionTris-Glycine SDS (TGS) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris, 0.192M Glycine, and 0.1% SDS. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Abcam Salsolinol hydrochloride, Dopamine derivative, 500MG
MW 215.68 g/mol, Purity >99%. Dopamine (ab120565) derivative. Putative endogenous opioid system-activating factor. Antagonizes L-DOPA-mediated hyperactivity. Shows central effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Teknova 50mM Tris, 16% Ethanol, pH 7.5. 1000mL. Endotoxin Tested, Sterile.
50mM Tris,16% (v/v) Ethanol
Biotium Fish Gelatin
Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies. Fish gelatin can be used in blocking buffers to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures.
Teknova 1M Tris-HCl, pH 7.6 Solution. 1000mL Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Alkali Scientific CellPro™ Tris Buffered Saline, 20X, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ Tris-HCL Buffer,1M, pH 8.0, 1L, 12/cs
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ Tris Buffer, 2M, pH 8.0, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific QuickSilver™ Buffer Powder, Tris-MOPS-SDS Buffer Powder, 50 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Bioworld Tris-Glycine-SDS (TG-SDS) Buffer, Ready-Pack (10X), 1 PK(s)
Tris Glycine-SDS BufferTD-SDS Buffer is blended using bioPLUS reagents to provide the highest quality buffer for protein research. Ideal for protein separations, this buffer is formulated to ensure exceptional purity in the absence of nuclease and protease activities.TG-SDS Buffer is a widely used running buffer for denaturing electrophoresis of proteins (SDS-PAGE).Technical Information:Storage: RTHazard: Irritant1 pack makes 1 L of 10x concentrate.1X Solution Contains: 0.025 M Tris, 0.192 M Glycine and 0.1% SDS.