Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 20L-CBY
Phosphate Buffered Saline PBS (pH 7 4 Sterile-Filtered) - 20L-CBY (For Research Use and Further Manufacturing Only)
Apexbio Technology LLC Annexin V-HF488/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds to double-stranded DNA emitting blue fluorescence and is generally unable to permeate intact cell membranes However during late apoptosis or cell death DAPI can penetrate compromised membranes enabling nuclear staining Therefore combined staining with Annexin V-HF488 and DAPI facilitates the differentiation of cells in different apoptosis stages as well as dead cells
Alkali Scientific CellPro™ PBS 10X, pH 7.0, 500mL, 500mL, 10/case
CellPro™ PBS 10X, pH 7.0, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Apexbio Technology LLC TUNEL Apoptosis Detection Kit (DAB) 20rxns
The TUNEL Apoptosis Detection Kit (DAB) is designed to detect nuclear DNA fragmentation occurring during apoptosis in tissues or cells During apoptosis endonucleases within cells become activated and cleave genomic DNA between nucleosomes resulting in DNA fragments of approximately 180-200 bp or multiples thereof The fragmented DNA generates free 3 -OH termini at which terminal deoxynucleotidyl transferase (TdT) incorporates biotin-labeled dUTP Subsequently these labeled termini bind to horseradish peroxidase (HRP)-conjugated streptavidin (Streptavidin-HRP) followed by chromogenic visualization using DAB This kit allows direct observation using an ordinary optical microscope and is convenient for use It is applicable to a wide range of sample types including frozen sections paraffin-embedded sections adherent cell cultures and suspended cells making it versatile for apoptosis detection
PLANT CELL TECHNOLOGY 1X Dulbecco's Phosphate Buffered Saline Solution without Calcium and Magnesium, Buffer Solution, Mod. 1, Clear Liquid, Sterile, 500mL bottle.
1X DPBS Mod. 1 is a sterile phosphate-buffered saline solution for mammalian cell culture applications. Provides isotonic environment to maintain cell integrity during washing, resuspension, or media preparation. Supplied as a clear liquid in a 500 mL sterile bottle. Ready-to-use and shelf stable under recommended conditions. Suitable for research, cell culture, and tissue engineering workflows.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.