Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 6.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 6.5 (0.2 micron filtered), 500 ml.
Boston Bioproducts Inc Molecular Biology Grade Water - 1L
Molecular Biology Grade Water - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Molecular Biology Grade Water - 500ml
Molecular Biology Grade Water - 500ml (For Research Use and Further Manufacturing Only)
Bioworld PEB- Protein Extraction Buffer (5x), 250 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
Medchemexpress LLC 1H-1,2,3-Triazole, 1-(3-iodophenyl)-4-(3-nitrophenyl)- | 1809031-84-2 | 99.5% | 392.15 | 10 MG
P62-mediated mitophagy inducer (PMI) is a P62-mediated mitophagy activator that activates mitochondrial autophagy without recruitment of Parkin or collapse of the mitochondrial membrane potential. It remains active in cells lacking a fully functional PINK1/Parkin pathway and serves as a pharmacological tool to study the molecular mechanisms of mitosis, avoiding toxicity and some of the non-specific effects associated with the sudden dissipation of mitochondria lacking membrane potential.
- P62-mediated mitophagy activator
- Activates mitochondrial autophagy without recruitment of Parkin or collapse of the mitochondrial membrane potential
- Remains active in cells lacking a fully functional PINK1/Parkin pathway
- Serves as a pharmacological tool to study the molecular mechanisms of mitosis
- Avoids toxicity and some non-specific effects associated with the sudden dissipation of mitochondria lacking membrane potential
