Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
- CAS Number: 77-86-1
- Molecular Formula: C4H11NO3
- Molecular Weight: 121.14 g/mol
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.6 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.6 (0.2 micron filtered), 500 ml.
Boston Bioproducts Inc Tris-Acetate-EDTA Buffer, TAE (50X) - 1L
The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.
Quality Biological Inc TE, pH 7.4 100mL
TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.
Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immuno-histochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Bioworld STE Buffer (10X), 4 L
STE ReagentCommon reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols.DNAse and RNAse free, 10X solutionTechnical Information:1X concentration solution containsNaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4) - 20L-CBY
Tris-HCl Buffer (1 M pH 7 4) - 20L-CBY (For Research Use and Further Manufacturing Only)
Bioworld TBE Urea Sample Buffer (2X), 50 mL
Product DescriptionTBE(Tris-Borate-EDTA) urea sample buffer is used for nucleic acid analysis. It has a 2X concentration and contains 45 mM Tris base, 45 mM Boric acid, 6% Ficol, 3.5M Urea, 0.005% Bromophenol blue, 0.025% Xylene and 1 mM EDTA.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 5kg
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 5kg
Alkali Scientific CellPro™ Tris Glycine SDS 10X, pH 8.3, 500 mL
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTA 5L
NC3626876 50 MM TRIS-HCL 10 MM EDTA 5L