Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 10mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes
Boston Bioproducts Inc PIPES Buffer (0.5 M, pH 7.5) - 100ml
PIPES Buffer (0 5 M pH 7 5) - 100ml (For Research Use and Further Manufacturing Only)
Research Products International Corp Blot Stripping Buffer 4X Solution, 250 ML
Product Formulation: TRIS-HCl: 250 mM; SDS: 8%; pH: 6.8. Add mercaptoethanol according to protocol.
Instructions :
1. Dilute 4X Stripping Bufffer ( 1part Stripping Buffer: 3 parts distilled water) and add beta-mercaptoethanol (0.6%).
2. Wash the wet membrane in the stripping buffer for 5 minutes.
3. Incubate the membrane in stripping buffer for 30 minutes at 65 degrees Celius while agitating.
Sigma Aldrich Fine Chemicals Biosciences BICINE Redi DriTM 99 free 500G
BICINE Redi DriTM 99 free 500G
Bioworld MOPS-SDS Running Buffer (20X), 1 L
MOPS-SDS Running Buffer for Protein Gel electrophoresisSDS running buffer with MOPS and Tris (pH 7.7)20X concentrationAll bioWORLD running buffers are prepared with high quality reagentsSpecifications:Ready to use, just dilute in distilled water. Save time and ensure reproducible results.1X contains: 50mM MOPS, 50mM Tris base, 1mM EDTA, and 0.1% SDS
Teknova 10X HBS-P Buffer, pH 7.4 at 1X. 1000mL, Sterile
1.5M Sodium Chloride,0.1M HEPES,0.5% Tween-20
Teknova 10X PBS Solution, pH 7.4 at 1X. 4L, Non-Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Alkali Scientific CellPro™ TE 1X (Tris EDTA), pH7.4, 100mL
Introducing CellPro™ TE 1X (Tris EDTA), meticulously formulated by Alkali Scientific™ to meet the exacting standards of molecular biology research. This Molecular Biology Grade TE buffer ensures exceptional purity and performance, making it an indispensable tool for various molecular biology applications, including DNA and RNA purification, DNA sequencing, and PCR. With its precisely balanced composition and stringent quality control measures, CellPro™ TE 1X provides researchers with reliable and reproducible results, facilitating accurate and efficient laboratory workflows. Trust CellPro™ TE 1X for your molecular biology experiments and experience the assurance of superior quality and performance in every assay.
Hampton Research HR2-243/StockOptions MES, pH 5.2 to 7.1 in 0.1 pH unit increments - 10 ml, tube format
HR2-243/StockOptions MES, pH 5.2 to 7.1 in 0.1 pH unit increments - 10 ml, tube format. Please note items from this supplier are non-returnable.
Boston Bioproducts Inc Tris-EDTA Buffer, TE (20X, pH 8.0) - 250ml
The Tris-EDTA (TE) Buffer is a commonly used buffer solution in molecular biology, especially in processes involving nucleic acids. This buffer is composed of a 20X concentration of Tris, a buffering agent, and EDTA, which has the ability to prevent degradation of DNA and RNA by chelating magnesium or other divalent metal ions, and allows pH regulation within the range of 7.5-8.5. It is widely used often for DNA and RNA gel electrophoresis, and also for DNA automated sequencing.