Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules 1214727-57-7 | TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000165855.jpg-250.jpg)
eMolecules 1214727-57-7 | TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861
TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | 1214727-57-7 | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861
Chemscene ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16
ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16
Biotang Inc TRIS-Glycine-SDS Running Buffer (10X), 1000 ml
TRIS-Glycine-SDS Running Buffer (10X), 1000 ml.
Kd Medical Inc TRIS-HCL BUFFER 1M, PH 7.5, 1L
Used for molecular biology, protein chemistry, and biochemical applications. 1 Molar solution, pH 7.5. One 1 liter bottle.
Sigma Aldrich Fine Chemicals Biosciences Trizma base | 77-86-1 | MFCD00004679 | 100g
Trizma base | Purity: 99.9% | Mol Wt: 121.41 | 77-86-1 | MFCD00004679 | 100g
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 250g
BICINE | Purity: 99% | Mol Wt: 163.17 | 150-25-4 | MFCD00004295 | 250g
Sigma Aldrich Fine Chemicals Biosciences Tricine | 5704-04-1 | MFCD00004277 | 100 g
Tricine | Purity: ≥99% | Mol Wt: 179.17 | 5704-04-1 | MFCD00004277 | 100 g
Sigma Aldrich Fine Chemicals Biosciences Tricine | 5704-04-1 | MFCD00004277 | 25g
Tricine | Purity: ≥99% (titration) | MW:179.17 | 5704-04-1 | MFCD00004277 | 25g
Sigma Aldrich Fine Chemicals Biosciences CAPS >=99% | 1135-40-6 | MFCD00003837 | 6X100G
CAPS >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 6X100G
Sigma Aldrich Fine Chemicals Biosciences CAPS BioXtra, >=99% | 1135-40-6 | MFCD00003837 | 1KG
CAPS BioXtra, >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 1KG
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 25g
CAPS | Purity: 99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 25g
Sigma Aldrich Fine Chemicals Biosciences CHES BioXtra, >=99.0% (titration) | 103-47-9 | MFCD00003835 | 100G
CHES BioXtra, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 100G
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 25g
CAPS | Purity: 99% | MW: 221.32 | 1135-40-6 | MFCD00003837 | 25g
Sigma Aldrich Fine Chemicals Biosciences CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | 1135-40-6 | MFCD00003837 | 1KG
CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 1KG
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G