Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ TAE 50X (Tris-Acetate EDTA), 1L
Alkali Scientific™ CellPro™ TAE Tris Acetate EDTA is a fundamental reagent in nucleic acid electrophoresis, renowned for its exceptional buffering capacity and DNA solubilization properties. Designed to provide optimal performance and reproducibility, this solution enables precise separation and analysis of DNA fragments in agarose gel electrophoresis. Each batch undergoes rigorous sterile filtration to ensure purity and consistency, empowering researchers with confidence in their molecular biology experiments.
Sigma Aldrich Fine Chemicals Biosciences MOBS >=99% (titration) | 115724-21-5 | MFCD00674916 | 25G
MOBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 223.29 | 115724-21-5 | MFCD00674916 | 25G
![Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659865.jpg-250.jpg)
Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kg
BIS TRIS Propane (1,3 Bis(TRIS(hydroxymethyl)methylamino)propane) is a high-purity buffer available in 1 kg powder form. It is commonly used in biochemical and molecular biology applications where a stable pH is essential, particularly in the range of 7.0–9.0. BIS TRIS Propane is effective in protein electrophoresis, enzyme activity assays, and other applications requiring robust buffering in alkaline conditions. The large 1 kg size is ideal for high-throughput laboratories, providing a cost-effective solution for experiments requiring stable pH over extended periods. This buffer is widely used in proteomics, genomics, and pharmaceutical research, where precise pH control is necessary for reproducible results.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-12 Bis-Tris Preca
mPAGE 4-12% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Bioworld TT Buffer, Ready-Pack (10X), 2 L
Tris-Tricine BufferTris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more conventionally used buffers. TT Buffer allows for optimal resolution of low molecular weight proteins and elimantes the glycine front often found in gel utilizing TG-SDS and other such beffers. This improved running buffer allows for resolution of peptides as low as 5kD. TT buffer is also excellent for protein electrophoresis where resolved products will be transferred to a nylon membrane. Unlike glycine, tricine will not interfere with membrane protein sequencing. The 10X pack just requires the addition of distilled H2O to make 1.0 Liter of 10X liquid. A simple 10-fold dilution with water provides a 1X solution, that makes an excellent running buffer for most applications. Technical Information:Ultra PureGradeStorage: RTHazard: Irritant
Quality Biological Inc Tris Glycine Transfer Buffer (10X) (Transblotting Buffer 10X), 1000 mL
Tris Glycine Transfer Buffer at a 1X concentration with the addition of 20% methanol is used for electrophoretic transfer of proteins from denaturing polyacrylamide gel to a solid membrane i.e. Western blotting or Tris-glycine gel electrophoresis.
Storage Conditions: 15 °C to 30 °C
Limit exposure to light by wrapping in foil or storing in a cabinet
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase and Protease Tested
Max Shelf Life: 24 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
eMolecules 172647-87-9 | TERT-BUTYL 2-(HYDROXYMETHYL)INDOLINE-1-CARBOXYLATE | AstaTech | MFCD06411549 | 249.310 | C14H19NO3 | 95.000 | CC(C)(C)OC(=O)N1C(CO)Cc2ccccc12 | 1g | 559763973
TERT-BUTYL 2-(HYDROXYMETHYL)INDOLINE-1-CARBOXYLATE | AstaTech | 172647-87-9 | MFCD06411549 | 249.310 | C14H19NO3 | 95.000 | CC(C)(C)OC(=O)N1C(CO)Cc2ccccc12 | 1g | 559763973
LAB BIOREAGENTS LowBinding I Buffer 250ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.