Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, RNase Free) - 250ml
Phosphate Buffered Saline PBS (10X RNase Free) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS - 1L
Dulbecco s Phosphate Buffered Saline DPBS - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Succinate Buffer (0.2 M, pH 5.5) - 500ml
Succinate Buffer (0 2 M pH 5 5) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-P+ Buffer (1X, pH 7.4) - 4x500ml
HBS-P Plus Buffer (1X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.5) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 7 5) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 500ml
HEPES Buffer (1 M pH 7 6 pH Adjusted with KOH) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc TAPS Buffer (0.2 M, pH 8.5) - 100ml
TAPS Buffer (0 2 M pH 8 5) - 100ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Saponin Cell Permeating Solution (0.5% w/v in PBS) - 4x100ml
Saponin Cell Permeating Solution (0 5% w/v in PBS) - 4x100ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-EP+ Buffer (10X, pH 7.4) - 4x500ml
HBS-EP Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.8) - 500ml
Tris-HCl Buffer (1 M pH 6 8) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Base (1 M) - 2x1L
Bioprocess Grade - Tris-Base (1 M) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Krebs-Henseleit Buffer (with HEPES, without Calcium) - 500ml
Krebs-Henseleit Buffer (with HEPES without Calcium) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Bovine Serum Albumin, BSA (5%, TBST) - 250ml
Bovine Serum Albumin BSA (5% TBST) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Glycine-Native Running Buffer (10X) - 1L
Tris-Glycine-Native Running Buffer (10X) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (2X, Low Endotoxin) - 250ml
HEPES Buffered Saline (2X Low Endotoxin) - 250ml (For Research Use and Further Manufacturing Only)