Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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2,821 – 2,835 of 3,500 results

2,821

Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4, Low Endotoxin) - 250ml

The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration and tested for endotoxin.

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2,822

Medchemexpress LLC Pipes dipotassium | 108321-27-3 | MFCD00069751 | 98.0% | 378.55 | C8H16K2N2O6S2 | 100 G

PIPES (dipotassium) is the dipotassium salt of the zwitterionic buffering agent PIPES, provided as a white to off-white solid. It offers stable buffering near physiological pH with low metal ion binding, making it suitable for biochemical and cell biology applications such as assay buffers, electrophoresis, and sample preparation.

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2,823

LEINCO TECHNOLOGIES INC PBS 1X STERILE 4X4L

NC1983347 PBS 1X STERILE 4X4L

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2,824

Biotang Inc CHES Buffer 0.2M, pH 8.5, 100 ml

CHES Buffer 0.2M, pH 8.5, 100 ml.

Pricing & Availability

2,825

Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 100G GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade

Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade , multicompendial