Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 7.0, 100 mL
Synonym:N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications. The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :Useful pH range :6.4 - 7.8pKa (25 °C) :7.1 (Free acid)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
Teknova MES Dilution Buffer, pH 6.5. 1000mL, Sterile 8 pack.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
Bioworld BES Buffer, 0.5M, pH 7.5, 500 mL
Product DescriptionBES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) buffer has a concentration of 0.5M and a pH value of 7.5. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Crescent Chemical Co Inc HYDROXYETHYL)PIPERAZINE-N'-2-E
Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid -Na-salt analytical grade pKa 20 = 7.55. Buffering substance for biochemistry and molecular biology. Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 25249.04 CAS 75277-39-3
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660020.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 25 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a high-quality biochemical reagent used for pH buffering in laboratory research. This 25g package of MOPS is ideal for small-scale laboratory applications that require a stable pH range of 6.5 to 7.9. MOPS is widely used in molecular biology, protein chemistry, and biochemical assays where maintaining precise pH conditions is essential. It is commonly used in enzyme studies, electrophoresis, and protein purification processes. The small packaging size is suitable for labs with lower buffer requirements or for preparing small quantities of buffer solutions for specific experiments.
![Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660311.jpg-250.jpg)
Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 25 G
HEPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)) is a biochemical reagent available in a 25g bottle. This buffering agent is ideal for laboratory applications where maintaining a stable pH is crucial. HEPPS is commonly used in enzyme assays, protein studies, and biochemical experiments, particularly those requiring precise control of pH. The reagent is also utilized in protein purification, electrophoresis, and other applications in molecular biology and biochemistry. Its ability to maintain pH stability at physiological conditions makes it essential for accurate, reliable results in biological research and testing.
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660135.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 100 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a biochemical reagent used for pH buffering in laboratory applications. This 100g package of MOPS is commonly used in electrophoresis, chromatography, and enzyme assays to maintain a stable pH in the range of 6.5 to 7.9, which is ideal for many biological processes. It is an important component in buffers for protein, nucleic acid, and enzyme studies. MOPS is particularly useful for experiments that require precision in maintaining a near-neutral pH, such as protein purification, electrophoresis, and molecular biology experiments. It is often chosen for its excellent buffering capacity, making it an essential reagent in many biochemical assays.
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660092.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 1 Kg
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a specialized biochemical reagent used for preparing buffer solutions that maintain a stable pH in laboratory experiments. This 1kg formulation is ideal for high-volume applications, including protein chemistry, enzyme assays, and microbiological studies. The sesquisodium salt form of PIPES offers enhanced buffering capacity and is commonly used in research that requires precise pH control, such as in cell culture, protein purification, and electrophoresis. The buffer solution maintains a stable pH around 7.0, which is essential for maintaining enzyme activity and cellular functions during experiments.
Bioworld Bicine, 500 g
A zwitterionic amino acid buffer agent used for low temperature work Technical Information:Synonym: N,N-Bis(2-hydroxyethyl)glycine Titration: > 98.0% (0.1N NaOH)Water content: < 2.0%Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.Appearance: PowderBoiling Point : 388.55 °C at 760 mmHgDensity : 1.05 g/cm3 at 20 °C (lit.)HS Code : 29225000Melting Point: 190 °C (dec.)(lit.)PH Value: 4.3-5.3 (1%)RTECS Number: MB9700000
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661641.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid) is a biochemical reagent used for pH buffering in biochemical applications. This 1 kg volume is ideal for laboratories requiring large quantities of MES buffer for a variety of experiments. MES is commonly used in biochemical research, especially in enzyme assays, cell culture, and protein purification. It maintains a stable pH range, crucial for experiments that need precise and consistent conditions. The MES buffer ensures minimal interference in reactions, making it ideal for applications such as electrophoresis and chromatography. MES Monohydrate is a reliable and effective reagent used in a wide range of scientific studies, particularly in molecular biology and biochemistry.
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 6.5, 500 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltpH: useful range: 6.0 - 7.2Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. PH Value: : 6.0 - 7.2
Crescent Chemical Co Inc MORPHOLINOPROPANE SULFONICACID
Morpholinopropane sulfonic acid analytical grade pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. Size - 500G Sto
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 24.5 Grams of Powder, Makes 5 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (5 liters, 24.5 grams of powder) is used for plant tissue culture applications that require precise control over pH levels and nutrient availability. The MES buffer stabilizes the pH environment, ensuring that plant cells receive consistent growth conditions. This medium is ideal for a variety of tissue culture applications, including shoot multiplication, regeneration, and plant propagation. The formulation includes essential vitamins and nutrients that support plant growth, making it an essential tool for researchers, plant breeders, and commercial growers. It is commonly used in agricultural laboratories and greenhouses to produce high-quality, healthy plant cultures.