Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
Phosphate buffered saline (PBS) is an isotonic buffer SOLUTIONon. This water-based salt SOLUTIONon is made up of disodium hydrogen phosphate sodium chloride and in some preparations potassium chloride and potassium dihydrogen phosphate. PBS is generally used in biological research.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate (Standard) | 145224-94-8 | 99.95% | 50 MG
MES monohydrate (Standard) is the analytical standard of MES (monohydrate), intended for research and analytical applications. MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments.
- Used for research and analytical applications.
- Amphoteric ion buffer.
- Buffer capacity ranging pH 5.5-7.0.
- Widely used in biochemistry and molecular biology experiments.
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS.
Hoefer, Inc MOPS-SDS RUNNING BUFFER 1LX10
Mighty Small precast gels Hoefer Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation.Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution. High Loading Capacity Maximum sample volume is 50 μl Good Repeatability Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability High Resolution Upgraded Bis-Trissystem for clearer, sharper separation bands and higher resolution Highly Effective Maximum 40 min for electrophoresis Ready to use and easy to store No solution preparation or gel casting is required, saving time. Store at 2-8°C Safe and non-toxic No hazardous reagents need to be handled Specifications Plate Size: 10×8 cmWells: 12-well, 15-well Concentration:• Gradient gels: 4-12ĉĉĉĉĉĉĉĉĉĉMOPS-SDS Running Buffer, 1L×10ĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉ27-Mar-25 07:45:08:000 PMĉ
Sigma Aldrich Fine Chemicals Biosciences HEPES buffered saline BioUltra, for molecular biology, 2x concentrate | MFCD00006158 | 50ML
HEPES buffered saline BioUltra, for molecular biology, 2x concentrate | MFCD00006158 | 50ML
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbecco Phosphate Buffered Saline (DPBS) is a balanced salt SOLUTIONon.
Research Products International Corp Triton Lysis Buffer Solution, 500 ML
1X buffer used to lyse cells under non-denaturing conditions.
1X Formulation:
NaCI: 150 mM
TritonX-100: 1%
EDTA: 5 mM
Abcam 10X ANNEXIN V/ANXA5 BINDING BU ..
10X ANNEXIN V/ANXA5 BINDING BU ..
The product is subject to the following: Abcam Restricted Use Statement
Hoefer, Inc MSML PRECSTGEL 10 BIS-TRIS 12W
Mighty Small precast gels Hoefer Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation.#11;Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution. High Loading Capacity Maximum sample volume is 50 μl Good Repeatability Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability High Resolution Upgraded Bis-Trissystem for clearer, sharper separation bands and higher resolution Highly Effective Maximum 40 min for electrophoresis Ready to use and easy to store No solution preparation or gel casting is required, saving time. Store at 2-8°C Safe and non-toxic No hazardous reagents need to be handled Specifications Plate Size: 10×8 cm#11;Wells: 12-well, 15-well Concentration:#11;• Gradient gels: 4-12%ĉĉĉĉĉĉĉĉĉĉMighty Small precast gels, 10% Bis-Tris, 12wellsĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉ27-Mar-25 07:45:08:000 PMĉ