Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences TRIZMA BASE ANHYDROUS 99.9%
Trizma® baseanhydrous, free-flowing, Redi-Dri™, ≥99.9%cas 77-86-1
Biotang Inc Lysis Buffer(His-tag protein purification from E.Coli and insect cells), 500 ml
Lysis Buffer(His-tag protein purification from E.Coli and insect cells), 500 ml.
eMolecules 5-Bromo-3-(hydroxymethyl)pyridin-2(1H)-one | 1227502-35-3 | MFCD30364462 | 250mg
Ambeed | 5-Bromo-3-(hydroxymethyl)pyridin-2(1H)-one | 250mg | 571782986 | A155880 | | 1227502-35-3 | MFCD30364462 | 204.023 | C6H6BrNO2
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Electron Microscopy Sciences Hepes, Crystal, Biological Buffer, 7.0-8.0 pH 100 GR
(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
CAS #7365-45-9 C₈H₁₈O₄N₂S F.W. 238.30
Assay: >99%
Good's buffer.
pH: 7.0-8.0
Sigma Aldrich Fine Chemicals Biosciences Tris Acetate-EDTA buffer B1L
TRIS Tris Acetate-EDTA buffer B1L
Supply Solutions Tris Buffered Saline, with Tween 20, pH 8.0, 10 pack
Tris Buffered Saline, with Tween 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay.
S2 Media 1M Tris-HCl, pH 8.0, Sterile, 1L Media Bottle, 1ea
1M Tris-HCl Buffer (sterile) is commonly used in labs for pH stabilization in biochemical reactions, as a buffer in analytical chemistry, and as a sample diluent near neutral to slightly basic pH. At pH 8.0, Tris (pKa ≈ 8.1 at 25°C) provides effective buffering between pH 7–9, making it compatible with most biological systems and minimally reactive with biomolecules.• 1M Tris-HCl should be used by trained professionals within a laboratory setting. • Tris is an abbreviation for tris(hydroxymethyl)aminomethane).• Product is not intended for use in the diagnosis or treatment of disease or other human conditions.• For Research Use Only (RUO). Not for use in diagnostic procedures.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML