Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences PIPES sodium salt >=99% (titration) | 10010-67-0 | MFCD00065472 | 500G
PIPES sodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 324.35 | 10010-67-0 | MFCD00065472 | 500G
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML
Sigma Aldrich Fine Chemicals Biosciences CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | 1135-40-6 | MFCD00003837 | 1KG
CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 1KG
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 100G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 100G
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 25g
CAPS | Purity: 99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 25g
Sigma Aldrich Fine Chemicals Biosciences CAPS >=99% | 1135-40-6 | MFCD00003837 | 6X100G
CAPS >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 6X100G
Sigma Aldrich Fine Chemicals Biosciences CHES BioXtra, >=99.0% (titration) | 103-47-9 | MFCD00003835 | 100G
CHES BioXtra, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 100G
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
Phosphate buffered saline (PBS) is an isotonic buffer SOLUTIONon. This water-based salt SOLUTIONon is made up of disodium hydrogen phosphate sodium chloride and in some preparations potassium chloride and potassium dihydrogen phosphate. PBS is generally used in biological research.
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer working solution, MFCD00236358
Tris-Borate-EDTA buffer working solution
Sigma Aldrich Fine Chemicals Biosciences HEPES, 7365-45-9, MFCD00006158, 100g
Empirical Formula (Hill Notation) C8H18N2O4S, ≥99.5% (titration), Molecular Weight 238.30, Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Sigma Aldrich Fine Chemicals Biosciences Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting, MFCD00236359
Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting