Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Ethos Biosciences Buffer tris multipurpose classic 1/EA
Dilurex 1M Tris-HCL 10X Buffer is a classic laboratory buffer at pH 7 4 It does not include a saline ionic component and is free to be mixed with other components as needed in your laboratory kip the tedium of weighing powders and stocking raw materials that can expire Our buffer is convenient ready to use and will save you hours in the lab letting you focus on breakthroughs rather than buffer prep Thousands of researchers trust Ethos Biosciences buffers for their proven track record in top-tier publications and protocols - upgrade your productivity today
Medchemexpress LLC MES hydrate | 1266615-59-1 | MFCD00149409 | 99.9% | 10 G
MES (2-Morpholinoethanesulphonic acid) hydrate is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is classified as a Good's buffer and is widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15. Its pKa value changes with temperature, a factor to consider when used at varying temperatures. MES hydrate exhibits good buffering properties in biological experiments and has a poor affinity for metal ions, making it suitable for studies involving such ions.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at approximately pH 6.15
- Poor affinity with metal ions
Medchemexpress LLC Mops (sodium salt) | 71119-22-7 | 99.83% | 10 G
The product is a buffering agent utilized in biology. It serves to maintain the pH of mammalian cell culture media and notably enhances the thermal stability of BSA.
- Functions as a buffering agent in biological applications.
- Maintains the pH level of mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Can be used in the preparation of keratinocyte and microbial culture medium.
- Relatively safe for short-term experiments, though prolonged use may reduce cell viability.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This modification has been shown to improve the stability of the medium.
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-250.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 250 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Research Products International Corp PIPES FREE ACID ULTRAPURE, 10GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | C29H33N3O9S2 | 10 MG
AR-13324 analog mesylate is a research-grade mesylate salt analog of AR-13324, a Rho-associated kinase (ROCK) and norepinephrine transporter (NET) inhibitor. It is supplied as a light brown to brown liquid intended for laboratory research and assay development.
- Research-use compound related to ROCK and NET inhibition.
- High purity (98.0%) suitable for analytical and biological assays.
- Molecular weight 631.72 g/mol for formulation calculations.
- Supplied as a mesylate salt in a liquid form for easy handling.
- Store sealed, away from moisture; in solvent: -80°C for 6 months, -20°C for 1 month.
Abcam Antigen Retrieval Buffer (100 x Citrate Buffer), 125ML
Antigen retrieval 100X citrate buffer (pH 5.1 - 5.5) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. - Cited in over 25 publications - Contains citrate buffer, 1.5% 2-butoxyethanol, 1% hydrochloric acid - Optimized for citrate buffer-treated antibodies - Contains detergent for paraffin emulsification
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 5 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, particularly in the preparation of enzyme buffers.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Especially useful in the preparation of enzyme buffers
GenDEPOT TBST(10X), pH 7.4 | 1L
TBST(10X) is a space-saving stock solution that is ideal for preparing TBS-Tween (TBS-T) wash buffers or blocking buffer diluent used for applications such as ELISA, Western blotting and other immunoassays. The 10X TBST (Tris-buffered saline with Tween™ 20 detergent) makes 25 mM Tris, 0.14M NaCl, 0.1% Tween-20, pH 7.4, when diluted to 1X with water.
Electron Microscopy Sciences Pipes 1.5 Sodium Salt, Crystal, 6.8 @25°C pH 100 GR
(Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium)
F.W. 335.3
pH: 6.8 @25°C