Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Thermo Scientific Production Chemicals and Services 50MM TRIS-HCL 10MM EDTA 0.6M
NC3588268 50MM TRIS-HCL 10MM EDTA 0.6M
Gold Biotechnology Inc HEPES, Free Acid 250 g
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660682.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L
TBE 10X Solution is an essential buffer used in the electrophoresis of nucleic acids such as DNA and RNA. This 4-liter bottle contains a concentrated mixture of Tris, borate, and EDTA, which provides the necessary pH and ionic environment for the proper migration of nucleic acid samples. TBE is particularly effective for separating small DNA fragments and is widely used in both research and clinical laboratories. By diluting the 10X stock to 1X, laboratories can prepare a buffer suitable for use in standard electrophoresis procedures. It is an important reagent for molecular biology applications requiring high-purity separation of nucleic acids.
Medchemexpress LLC 1H-1,4,7-triazonine-1,4,7-triacetic acid, α1-(2-carboxyethyl)hexahydro-, tris(t-Bu) ester | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 100 MG
NODAGA-tris(t-Bu ester) is a tri-tert-butyl ester-protected NODAGA chelator intended for conjugation to biomolecules and subsequent radiolabeling for positron emission tomography (PET) studies. It provides a protected, bifunctional scaffold that facilitates attachment to peptides, proteins, and antibodies and enables stable chelation of radiometals after deprotection.
- Protected NODAGA chelator for conjugation to biomolecules.
- Suitable for labeling peptides, proteins, and antibodies.
- Designed for radiolabeling workflows for PET imaging.
- High purity (99.8%) with RP-HPLC documentation available.
- Available as solid and as DMSO solutions for convenience.
- Molecular formula C27H49N3O8; molecular weight 543.69 g/mol.
![eMolecules 1357351-84-8 | tert-butyl 4-(hydroxymethyl)-8-azaspiro[4.5]decane-8-carboxylate | MFCD22209538 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502156925.JPG-250.jpg)
eMolecules 1357351-84-8 | tert-butyl 4-(hydroxymethyl)-8-azaspiro[4.5]decane-8-carboxylate | MFCD22209538 | 1g
Ambeed | Methyl 7-Methylindole-2-carboxylate | 250mg | 600840921 | A461257 | 16732-82-4 | MFCD04972090 | 189.214 | C11H11NO2
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eMolecules 279255-90-2 | tert-Butyl 5-(hydroxymethyl)-1H-indole-1-carboxylate | Combi-Blocks, Inc. | MFCD09834884 | 247.294 | C14H17NO3 | 98.000 | CC(C)(C)OC(=O)n1ccc2cc(CO)ccc12 | 1g | 603139295
tert-Butyl 5-(hydroxymethyl)-1H-indole-1-carboxylate | Combi-Blocks, Inc. | 279255-90-2 | MFCD09834884 | 247.294 | C14H17NO3 | 98.000 | CC(C)(C)OC(=O)n1ccc2cc(CO)ccc12 | 1g | 603139295