Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld EGTA 0.5M, PH 8.0,250ML
EGTA 0.5M, pH 8.0, DNase and RNase Free,250ml.EGTA Buffer is of a 0.5M concentration and pH of 8.0 that is DNase and RNase free. EGTA Buffer is a metal chelating agent specific for calcium ions. EGTA Buffer is commonly used in tissue culture media and biological buffers.
FUJIFILM BIOSCIENCES INC PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Abcam E x traction Buffer 5 x PTR, 50ML
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
The product is subject to the following: Abcam Restricted Use Statement
Genscript Corporation Tris-MOPS-SDS Running Buffer Powder
Tris-MOPS-SDS Running Buffer Powder are used for ExpressPLUS and SurePAGE gel transfer. Using proprietary techniques, Tris-MOPS-SDS Running Buffer Powder are made to have long shelf life, high resolution, fast electrophoresis, smaller volume.
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
United States Biological Corporation TRIS BASE ULTRAPURE 1KG
Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Purity: ≥99.9% Appearance: White, crystalline powder pH (1M): 10.5-11.5 pKa: 8.0-8.4 Absorbance (260nm): ≤0.06 Absorbance (280nm): ≤0.05 Absorbance (290nm): ≤0.2 Solubility (1M): Colorless, clear, complete Loss on Drying: ≤0.2% Melting Point: 168-172°C Heavy Metals (Pb): ≤0.0001% Arsenic: ≤0.0001% Calcium: ≤0.0001% Copper: ≤0.0001% Iron: ≤0.0005% Magnesium: ≤0.0001% Insoluble Matter: ≤0.005% Residue on Ignition: ≤0.1% RNase: DNase: Protease: Meets specifications.
Boston Bioproducts Inc MES Buffer (1 M, pH 6.0) - 500ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 100ML
Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 100ML
Teknova 50mM Tris, 150mM Sodium Chloride. pH 7.5, 1000mL. Endotoxin Tested. Sterile.
50mM Tris, 150mM Sodium Chloride. pH 7.5, 1000mL. Endotoxin Tested. Sterile.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (Sterile-Filtered, RNase Free) - 1L
Phosphate Buffered Saline (PBS) is a buffer solution widely used in biological research due to its isotonic and non-toxic nature. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for applications including protein purification, western blotting and immunoassays, sample dilution, and as a transport medium. This buffer is also tested for RNase enzymes, minimizing risk of nucleic acid degredation in downstream applications.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, MFCD00132476, 10pk
pH 8.0, powder, Synonym: TBS. Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); pH 8.0, at 25 °C.
Boston Bioproducts Inc Glycine Buffer in PBS (0.2 M, pH 2.5) - 500ml
The Glycine Buffer in PBS is widely used in various laboratory applications such as polyacrylamide gel electrophoresis as a running buffer, a transfer buffer for Western blotting, enzyme assays, and cell culture. This Buffer type serves as a tool for maintaining stable conditions during biochemical experiments in the pH range of 2.2 to 3.6. The Glycine buffer is an essential part of many types of protein purification procedures, including the purification and recovery of monoclonal antibodies.
Sigma Aldrich Fine Chemicals Biosciences Trizma hydrochloride, 1185-53-1, MFCD00012590, 500 g
Linear Formula: NH2C(CH2OH)3 · HCl, Molecular Weight: 157.60, BioPerformance Certified, cell culture tested, ≥99.0% (titration), Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride.