Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate | 145224-94-8 | MFCD00149409 | 500g
MES monohydrate | Purity: 99% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 500g
Supply Solutions Tris Buffered Saline, with Tween 20, pH 8.0, 10 pack
Tris Buffered Saline, with Tween 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 500g
BIS-TRIS | Purity: 98% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 500g
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 1KG
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 1KG
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS >=98.0% (titration) | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS >=98.0% (titration) | Purity: >=98.0% (titration) | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Rockland Immunochemicals Normal Saline - MB-049-1000
This solution is commonly referred to as physiological saline or isotonic saline.
Research Products International Corp Mops Sodium Salt, 100G, 71119-22-7, MFCD00064350
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Sigma Aldrich Fine Chemicals Biosciences MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | 1132-61-2 | MFCD00006183 | 1KG
MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | Purity: >=99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 1KG
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 100g
MOPS | Purity: 99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 100g
Growcells PBS 1X 10L EA
Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 8g, KCl 0.2g, Na2HPO4 1.44g, KH2PO4 0.24g
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioXtra, >=98.0% (titration) | 6976-37-0 | MFCD00002853 | 500G
BIS-TRIS BioXtra, >=98.0% (titration) | Purity: >=98.0% (titration) | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 500G
Sigma Aldrich Fine Chemicals Biosciences MOPS >=99.5% (titration) | 1132-61-2 | MFCD00006183 | 25G
MOPS >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 25G
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioXtra, >=98.0% (titration) | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioXtra, >=98.0% (titration) | Purity: >=98.0% (titration) | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Electron Microscopy Sciences Hepes Buffer Solution 0.2M, 7.0-8.0 pH 500 ML
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged. Desired pH should be specified when ordering.
pH: 7.0-8.0
Electron Microscopy Sciences Tris (Hydroxymethyl) Aminomethane Free Base, Reagent 100 GR
CAS #77-86-1 C₄H₁₁NO₃ F.W. 121.14
Assay: >99.9%
White crystalline powder. A 40% (w/v) aqueous solution. Hydroxymethyl Aminomethane.
Grade: Reagent